Figure 3.

lncRNA-MEG inhibit neuronal apoptosis through miR-21-5p in SCI rats. (A) Screen for siRNAs that effectively inhibit lncRNA-MEG in VSC4.1 cells. VSC4.1 cells were transiently transfected with different siRNAs, and the relative knockdown of lncRNA-MEG was detected by qPCR. The relative changes were normalized for the mock group. Each data point represents the mean ± SD of three independent experiments and was analyzed with T-test. *P < 0.05, **P < 0.01. (B, C) In the SCI rat model, the spinal cord tissues of rats were collected for detection after 7 days. No. 1 to No. 5 are control group and No. 6 to No. 10 are SCI model group. The expression of lncRNA-MEG (B) and miR-21-5p (C) in different groups of rats were analyzed by qPCR. (D) Apoptosis in rat spinal cord group was identified by Tunnel staining. Blue represents DAPI and red represents apoptotic cells. (E) The levels of indicated proteins were measured with western Blot after lncRNA-MEG knocking down with shRNA lentivirus in SCI rat spinal cord tissues.