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. 2021 Feb 15;13(2):565–584.

Figure 5.

Figure 5

The inhibitory effect of Tan IIA against fibroblast proliferation could be partially reversed with 3-MA. A. Fluorescence microscopy images of EdU-positive (green) fibroblasts after treatment with 3-MA and/or Tan IIA for 48 h. Merged images showed overlap of the nucleus (blue); magnification, ×100; scale bar, 10 μm. B. Proportion of EdU-positive cells. C. Cell cycle analysis of fibroblasts after treatment with 3-MA and/or Tan IIA for 48 h. D. Proportion of fibroblasts at G2/M phase. E-G. PCNA and cyclin D1 expression was assessed by western blot and quantified by normalization to GAPDH expression. Data are shown as the means ± SDs from three independent experiments; *P < 0.05; #P > 0.05.