Figure 6.

The suppressive effect of Tan IIA against fibroblast migration could be partially reversed with 3-MA. A, C. The inhibitory effects of Tan IIA against migration in fibroblasts were visualized with Transwell and Scratch wound experiments; scale bars, 50 and 100 μm. B. The number of migrated fibroblasts that attached to the bottom membrane after 3-MA and/or Tan IIA treatment for 48 h. D. Wound closure rate of cells treated with 3-MA and/or Tan IIA for 0, 12, 24, or 36 h. E. Confocal images of the cytoskeleton proteins tubulin and vinculin after treatment with 3-MA and/or Tan IIA for 48 h; scale bar, 10 μm. F-H. Tubulin and vinculin protein expression was confirmed by western blot and quantified by normalization to GAPDH expression. Data are shown as the means ± SDs of three independent experiments; *P < 0.05; #P > 0.05.