Figure 5.

G3BPs bridge TSC2 to LAMP proteins

(A) IP against TSC1 (TSC1 #1) or mock (rabbit IgG) in TSC2 KO cells. n = 3.

(B) IP against GFP or FLAG; transfection with the indicated plasmids. n = 5.

(C) IP against TSC1 (TSC1 #2) or mock (mouse IgG) incubated with NaCl or SDS. n = 3.

(D) IP against GFP or mock (mouse IgG); transfection with the indicated plasmids. n = 3.

(E) BiFC. Protein+C-terminal mLumin is indicated first; protein+N-terminal mLumin is indicated second. TL, transmitted light. Scale bar, 100 μm. n = 3.

(F) Quantitation of data in (E). Shown are data points and mean ± SEM.

(G) IP against MTOR or mock (rat IgG). n = 3.

(H) BiFC. Protein+C-terminal mLumin is indicated first; protein+N-terminal mLumin is indicated second. TL, transmitted light. Scale bar, 100 μm. n = 4.

(I) Quantitation of data in (H). Shown are data points and mean ± SEM.

(J) Quantitation of data in (K). Shown are data points and mean ± SEM.

(K) BiFC. Protein+C-terminal mLumin is indicated first; protein+N-terminal mLumin is indicated second. TL, transmitted light. Scale bar, 100 μm. n = 5.

(L) BiFC. Protein+C-terminal mLumin is indicated first; protein+N-terminal mLumin is indicated second. TL, transmitted light. Scale bar, 100 μm. n = 3.

(M) Quantitation of data in (L). Shown are data points and mean ± SEM.

(N) IP against FLAG or mock (mouse IgG); transfection with the indicated plasmids. n = 3.

(O) IP against FLAG or mock (mouse IgG); transfection with the indicated plasmids. n = 3.

(P) Phylogenetic analysis. Black square, protein present in species.

See also Figure S5.