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. 2021 Feb 4;184(3):655–674.e27. doi: 10.1016/j.cell.2020.12.024
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

CALR Cell Signaling Cat# 12238; RRID: AB_2688013
CANX Cell Signaling Cat# 2679; RRID: AB_2228381
CTSD Cell Signaling Cat# 2284; RRID: AB_10694258
EEA1 Cell Signaling Cat# 3288; RRID: AB_2096811
EIF2S1 Cell Signaling Cat# 9722; RRID: AB_2230924
EIF2S1-pS51 Cell Signaling Cat# 9721; RRID: AB_330951
EIF3A Cell Signaling Cat# 3411; RRID: AB_2096523
FLAG Sigma-Aldrich Cat# F3165; RRID: AB_259529
G3BP1 Santa Cruz Cat# sc-365338; RRID: AB_10846950
G3BP1 Santa Cruz Cat# sc-81940; RRID: AB_1123055
G3BP2 Bethyl Cat# A302-040A; RRID: AB_1576545
GAPDH Abcam Cat# ab37187; RRID: AB_732651
GAPDH (zebrafish) Sigma-Aldrich Cat# SAB2701826
GFP Roche Cat# 11814460001; RRID: AB_390913
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat# A-11001; RRID: AB_2534069
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat# A-11011; RRID: AB_143157
Goat anti-Mouse IgG (H+L) cross-adsorbed secondary, Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21422; RRID: AB_2535844
Goat anti-Rabbit IgG (H+L) cross-adsorbed secondary, Alexa Fluor 488 Thermo Fisher Scientific Cat# A-11008; RRID: AB_143165
Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP-coupled Thermo Fisher Scientific Cat# 31430; RRID: AB_228307
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP-coupled Thermo Fisher Scientific Cat# 31460; RRID: AB_228341
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Dylight 800 (zebrafish) Thermo Fisher Scientific Cat# SA5-35571; RRID: AB_2556775
Goat anti-Rat IgG (H+L) Secondary Antibody, HRP-coupled Thermo Fisher Scientific Cat# 31470; RRID: AB_228356
GOLGA1 Cell Signaling Cat# 13192; RRID: AB_2798144
GOLGA2 Cell Signaling Cat# 12480; RRID: AB_2797933
HA Roche Cat# 11867423001; RRID: AB_390918
Histone H3 (H3C1) Bethyl Cat# A300-822A; RRID: AB_597872
HSP60 (HSPD1) Cell Signaling Cat# 12165; RRID: AB_2636980
HSP90 (CDC37) Cell Signaling Cat# 4877; RRID: AB_2233307
LMNA A/C Cell Signaling Cat# 2032; RRID: AB_2136278
LAMP1 Cell Signaling Cat# 9091; RRID: AB_2687579
LAMP1 Developmental Studies Hybridoma Bank Cat# H4A3; RRID: AB_2296838
LAMP2 Cell Signaling Cat# 49067;RRID: AB_2799349
LAMP2 Santa Cruz Cat# sc-18822; RRID: AB_626858
LAMP2 Developmental Studies Hybridoma Bank Cat# H4B4; RRID: AB_2134755
MTOR Cell Signaling Cat# 2983; RRID: AB_2105622
MTOR epitope maps to residues 221 and 261 of human mTOR Monoclonal Antibody Core Unit.
Helmholtz Center Munich, Germany
TQREP-3G6
Mock antibody mouse Santa Cruz Cat# sc-2025; RRID: AB_737182
Mock antibody rabbit Bethyl Cat# P120-101; RRID: AB_479829
Sigma-Aldrich Cat# I5006; RRID: AB_1163659
Mock antibody rat Monoclonal Antibody Core Unit.
Helmholtz Center Munich, Germany
RmC3-7H8
Mouse IgG HRP Linked Whole Ab Merck Cat# GENA931
MYC-tag Cell Signaling Cat# 2276; RRID: AB_331783
Rabbit IgG HRP Linked Whole Ab Merck Cat# GENA934; RRID: AB_2722659
RPS6KB1 Cell Signaling Cat# 2708; RRID: AB_390722
RPS6KB1-pT389 Cell Signaling Cat# 9206; RRID: AB_2285392
RPS6KB1-pT389 Cell Signaling Cat# 9205; RRID: AB_330944
RAB5A Cell Signaling Cat# 3547; RRID: AB_2300649
RAB7A Cell Signaling Cat# 9367; RRID: AB_1904103
RPTOR Cell Signaling Cat# 2280; RRID: AB_561245
RPTOR #1 epitope maps to residues 686 and 704 of human Raptor Monoclonal Antibody Core Unit.
Helmholtz Center Munich, Germany
RAP1-20C4
RPTOR #2 Bethyl Cat# A300-553A; RRID: AB_2130793
RPS6 Cell Signaling Cat# 2317; RRID: AB_2238583
RPS6-pS235/236 Cell Signaling Cat# 4856; RRID: AB_2181037
RPS6-pS235/236 (zebrafish) Cell Signaling Cat# 2211; RRID: AB_331679
RPS6-pS235/236 (zebrafish) Cell Signaling Cat# 4858; RRID: AB_916156
TSC1 Cell Signaling Cat# 4906; RRID: AB_2209790
TSC1 #1 Gift from Michael N. Hall, Basel, Switzerland (Molle, 2006). Generated according to van Slegtenhorst et al. (1998). N/A
TSC1 #2 Thermo Fisher Scientific (Invitrogen) Cat# 37-0400; RRID: AB_2533292
TSC1 #3 Cell Signaling Cat# 6935; RRID: AB_10860420
TSC2 Cell Signaling Cat# 4308; RRID: AB_10547134
TSC2 #1 Thermo Fisher Scientific (Invitrogen) Cat# 37-0500; RRID: AB_2533293
TSC2 #2 epitope maps to residues 1535 and 1784 of human TSC2 Gift from Michael N. Hall, Basel, Switzerland (Molle, 2006). Generated according to van Slegtenhorst et al. (1998). N/A
TSC2 #3 Abcam Cat# ab52936; RRID: AB_883283
TUBA1B Abcam Cat# ab108629; RRID: AB_10866252
VDAC Cell Signaling Cat# 4661; RRID: AB_10557420
For antibody dilutions, see Table S3

Bacterial and Virus Strains

DB3.1 Thermo Fisher Scientific Cat# 11782018 (discontinued)
DH5-alpha New England Biolabs Cat# C2987H

Chemicals, Peptides, and Recombinant Proteins

AEBSF (4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride) Sigma-Aldrich Cat# A8456
Aprotinin Sigma-Aldrich Cat# A1153
Benzamidine Sigma-Aldrich Cat# B6506
Beta-Mercaptoethanol GIBCO Cat# 21-985-023
Bromophenol Blue Sigma-Aldrich Cat# B5525
BSA (bovine serum albumin) Carl Roth Cat# 8076.5
Calcein AM Fluorescent Dye Corning Cat# 354216
CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate) Sigma-Aldrich Cat# 3023
CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate)
(for IPs in rat brain tissue)
Roth Cat# 1479.3
Complete Protease Inhibitor Cocktail Sigma-Aldrich Cat# D27802
DABCO (1,4-diazabicyclo[2.2.2]octane) Merck Cat# 11836153001
DexoMAG® Liquids Research N/A
DMEM (Dulbecco’s Modified Eagle’s Medium) w: 4.5 g/L Glucose, w/o: L-Glutamine, w: Sodium pyruvate, w: 3.7 g/L NaHCO3 PAN Cat# P04-03600
DMEM (Dulbecco’s Modified Eagle’s Medium) used for PLA experiments in Figure 4A Thermo Fisher Scientific Cat# 41965-039
DMEM (Dulbecco’s Modified Eagle’s Medium) used for lysosomal isolations and trypsin digests Thermo Fisher Scientific Cat# 41965-062
DMSO (dimethyl sulfoxide) Sigma-Aldrich Cat# D2650
Doxycycline Sigma-Aldrich Cat# D3447
Duolink In Situ Mounting Medium with DAPI Sigma-Aldrich Cat# DUO82040
Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat# 10009D
E64 Sigma-Aldrich Cat# E3132
Ethosuximide Sigma-Aldrich Cat# 1001096708
FBS (fetal bovine serum) GIBCO Cat# 10270106
FBS (fetal bovine serum) Sigma-Aldrich Cat# F9665
Glycerol Sigma-Aldrich Cat# G5516
Glycine Sigma-Aldrich Cat# G7126
HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) Life technologies Cat# 15630080
HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) Life technologies Cat# 15630106
HBSS (Hank’s Balanced Salt Solution) w/o: Phenol red, w: Ca and Mg, w: 0.35 g/L NaHCO3 PAN Cat# P04-32505
Hoechst 33342 (dilution in IF: 1:100,000) Invitrogen Cat# H3570
Insulin Sigma-Aldrich Cat# I1882
IGEPAL CA-630 (NP40) Sigma-Aldrich Cat# I8896
Imidazole Sigma-Aldrich Cat# I0250
KCl (potassium chloride) Sigma-Aldrich Cat# P9541
Leupeptin Sigma-Aldrich Cat# 103476-89-7
L-glutamine GIBCO Cat# 25030024
L-glutamine GIBCO Cat# 25030081
Methanol Klinipath Cat# 4063-9005
MgCl2 (magnesium chloride) Sigma-Aldrich Cat# M2670
MK2206 Axon Medchem Cat# 1684
Mowiol 4-88 Carl Roth Cat# 07131
NaCl (sodium chloride) Sigma-Aldrich Cat# S7653
NPG (n-propyl-gallate) VWR Cat# EM8.20599.0500
PBS (phosphate-buffered saline) PAN Cat# P04-36500
PBS (phosphate-buffered saline) for non-sterile washing Biochrom Cat# L182-50
Penicillin/Streptomycin GIBCO Cat# 15140122
Pepstatin A Sigma-Aldrich Cat# 26305-03-3
PhosSTOP for IPs in brain tissue Merck Cat# 4906837001
Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726
Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044
Phusion HF DNA Polymerase Roche Cat# M0530S
Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Scientific Cat# 28908
Polyacrylamide Bio-Rad Cat# 161-0159
Polybrene Sigma-Aldrich Cat# H9268
Prolong Gold antifade reagent with 4′,6-Diamidin-2-phenylindol (DAPI) Thermo Fisher Scientific Cat# P36935
Protein G Sepharose beads GE Cat# 17061801
Puromycin Sigma-Aldrich Cat# P8833
PVDF (polyvinylidene difluoride) membrane Millipore Cat# IPVH00010
Rapamycin Calbiochem Cat# 553210
SDS (sodium dodecyl sulfate) Sigma-Aldrich Cat# 71725
Sodium deoxycholate Sigma-Aldrich Cat# 30970
Sodium fluoride Sigma-Aldrich Cat# 7681-49-4
Sodium glycerophosphate Sigma-Aldrich Cat# G9322
Sodium orthovanadate Sigma-Aldrich Cat# 13721-39-6
Sodium pyrophosphate Sigma-Aldrich Cat# 13472-36-1
Sucrose Sigma-Aldrich Cat# S2395
T4 DNA Ligase New England Biolabs Cat# M0202S
Torin1 Axon Medchem Cat# 1833
Transfectin Biorad Cat# 1703350
Tricaine (MS-222) Sigma-Aldrich Cat# E10521
TRIS base (tris(hydroxymethyl)aminomethane) VWR Cat# A1086.5000
Triton X-100 Sigma-Aldrich Cat# 93443
Trypan Blue GIBCO Cat# 15250061
Trypsin GIBCO Cat# 15400054
Tween-20 MP Biomedicals Cat# 11TWEEN201

Critical Commercial Assays

Bio-Rad Protein Assay Dye Reagent Concentrate Bio-Rad Cat# 500-0006
Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat# DUO92008
Duolink® In Situ PLA® Probe Anti-Rabbit PLUS Affinity purified Donkey anti-Rabbit IgG (H+L) Sigma-Aldrich Cat# DUO92002; RRID: AB281940
Duolink® In Situ PLA® Probe Anti-Mouse MINUS Affinity purified Donkey anti-Mouse IgG (H+L) Sigma-Aldrich Cat# DUO92004; RRID: AB_2713942
JetPEI Poly-Plus Cat# 101-40N
Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000015
Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778150
MidiPrepKit NUCLEOBOND XTRA MIDI Macherey-Nagel Cat# 740410.50
NuPage MES SDS running buffer Thermo Fisher Scientific Cat# NP0002
NuPage Novex 10% Bis-Tris gel Thermo Fisher Scientific Cat# NP0302BOX
Odyssey blocking buffer Li-Cor Cat# 927-40000
Pierce BCA protein assay kit Thermo Fisher Scientific Cat# 23225
Pierce ECL Western Blotting Substrate Thermo Fisher Scientific Cat# 32209
RIPA buffer (zebrafish lysis) Merck Cat# R0278
SuperSignal West FEMTO Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34095
Trans-Lentiviral shRNA Packaging Mix Dharmacon Cat# TLP5912

Deposited Data

Invasive breast cancer (The Cancer Genome Atlas, TCGA, provisional) http://www.cbioportal.org RRID: SCR_014555
TSC1 RNA expression data http://www.kmplot.com probeID: 209390_at
TSC2 RNA expression data http://www.kmplot.com probeID: 215735_s_at
G3BP1 RNA expression data http://www.kmplot.com probeID: 225007_at
G3BP1 protein expression data http://www.kmplot.com probeID: Q13283

Experimental Models: Cell Lines

HEK293T Thien et al. (2015) N/A
HEK293T Control This paper N/A
HEK293T G3BP1 KO This paper N/A
HEK293-β2AR Lavoie et al. (2002) N/A
HeLa alpha Kyoto Thedieck et al. (2007) N/A
HeLa S3 ATCC Cat# CCL-2.2; RRID: CVCL_0058
MCF-7 ACC115 DSMZ Cat# ACC115; RRID: CVCL_0031
MCF-7 Control (for G3BP1) This paper N/A
MCF-7 Control (for TSC2) This paper N/A
MCF-7 G3BP1 KO This paper N/A
MCF-7 GFP-LC3 Gift from Joern Dengjel, Fribourg, Switzerland N/A
MCF-7 shControl This paper N/A
MCF-7 shG3BP1 #1 This paper N/A
MCF-7 shG3BP1 #2 This paper N/A
MCF-7 TSC2 KO This paper N/A
MDA-MB-231 ATCC Cat# HTB-26; RRID: CVCL_0062
MDA-MB-231 TSC Control This paper N/A
MDA-MB-231 TSC2 KO This paper N/A
MDA-MB-231 shControl This paper N/A
MDA-MB-231 shG3BP1 #1 This paper N/A
MDA-MB-231 shG3BP1 #2 This paper N/A

Experimental Models: Organisms/Strains

AB Danio rerio Zebrafish International Resource Center Cat# ZL1; RRID: ZIRC_ZL1
Tg(dlx5a/dlx6a-EGFP) x Tg(vglut2a:loxP-RFP-loxP-GFP) Noble et al. (2015)Tg(dlx5a/dlx6a-EGFP);
Satou et al. (2012)Tg(vglut2a:loxP-RFP-loxP-GFP)
N/A
Tg(HuC:GCaMP5G) Ahrens et al. (2013) N/A
Wistar Cmd:(WI)WU rats Mossakowski Medical Research Centre Polish Academy of Sciences N/A

Oligonucleotides

control MO: 5′-CCTCTTACCTCAGTTACAATTTATA-3′ GeneTools N/A
g3bp1 MO: 5′-TAACAAAGGGCAAGTCACCTGTGCA-3′ GeneTools N/A
tsc2 MO: 5′-CTGTGTGTTTTCTTACTGGACCATA-3′ GeneTools N/A
For primers, see Table S4 N/A
For RNA, see Table S5 N/A

Recombinant DNA

bFos-MYC-LC151 Gift from Qingming Luo, Wuhan, China (Chu et al., 2009) N/A
bJun-HA-LN151 Gift from Qingming Luo, Wuhan, China (Chu et al., 2009) N/A
lentiGuide-Puro Sanjana et al. (2014) RRID: Addgene_52963
pCMV6-AN-MYC-DDK (MYC-FLAG-empty) Origene Cat# PS100016
MYC-FLAG-G3BP1 This paper N/A
MYC-FLAG-G3BP1-ΔRGG (aa 410-466) This paper N/A
MYC-FLAG-G3BP1-ΔNTF2L (aa 1-139) This paper N/A
MYC-FLAG-G3BP1-NTF2L 1-139 This paper N/A
MYC-FLAG-G3BP2 This paper N/A
pCW-Cas9-Blast Sanjana et al. (2014) RRID: Addgene_83481
pGW-MYC-LC151 Stefan Pusch (Weiler et al., 2014) N/A
pGW-HA-LN151 Stefan Pusch (Weiler et al., 2014) N/A
pGW-HA-LN151-G3BP1 This paper N/A
pGW-MYC-LC151-G3BP1 This paper N/A
pGW-MYC-LC151-G3BP1 1-182 This paper N/A
pGW-MYC-LC151-G3BP1 183-332 This paper N/A
pGW-MYC-LC151-G3BP1 333-466 This paper N/A
pGW-HA-LN151-G3BP2 This paper N/A
pGW-MYC-LC151-G3BP2 This paper N/A
pGW-HA-LN151-LAMP1 This paper N/A
pGW-MYC-LC151-LAMP1 This paper N/A
pGW-MYC-LC151-LAMP1 1-382 This paper N/A
pGW-MYC-LC151-LAMP1 383-417 This paper N/A
pGW-HA-LN151-LAMP2 This paper N/A
pGW-HA-LN151-mTOR This paper N/A
pGW-HA-LN151-TSC2 This paper N/A
pEGFP-C-LAMP2 This paper N/A
pEGFP-C-TSC2 This paper N/A
pEGFP-C (derivate of pDEST with a C-terminal EGFP tag) Stefan Pusch N/A
pDEST Stefan Pusch; Clone repository of
the DKFZ Genomics and Proteomics Core Facility (GPCF)
N/A
pDONR201-tGFP This paper N/A
pDONR201-tGFP-LAMP1 383-417 This paper N/A
pENTR221-G3BP1 Clone repository of
the DKFZ Genomics and Proteomics Core Facility (GPCF)
CloneId: 182373397
pENTR223-G3BP2 Clone repository of
the DKFZ Genomics and Proteomics Core Facility (GPCF)
CloneId: 192451551
pENTR221-LAMP1 Clone repository of
the DKFZ Genomics and Proteomics Core Facility (GPCF)
CloneId: 193137117
pENTR221-LAMP2 Clone repository of
the DKFZ Genomics and Proteomics Core Facility (GPCF)
CloneId: 115072391
pSpCas9(BB)-2A-Puro (PX459) V2.0 Addgene RRID: Addgene_62988
psPAX2 Shalem et al. (2014) RRID: Addgene_12260
pMD2.G Shalem et al. (2014) RRID: Addgene_12259
R777-E138 Hs.MTOR-nostop Gift from Dominic Esposito, Addgene Cat# 70422; RRID: Addgene_70422
R777-E356 Hs.TSC2-nostop Gift from Dominic Esposito, Addgene Cat# 70640; RRID: Addgene_70640

Software and Algorithms

Adobe Photoshop version CS5.1 Adobe Systems Incorporated RRID: SCR_014199;https://www.adobe.com/de/products/photoshop.html#
Bash script to identify the single nucleotide variants (SNV) within G3BP1 and G3BP2 This paper Document S3
Cell Profiler version 3.1.5 McQuin et al. (2018) RRID: SCR_007358;
https://www.cellprofiler.org/
CGDS-R package version 1.2.6 Gao et al. (2013) https://github.com/cBioPortal/cgdsr
Dell Statistica version 13 Dell Inc. https://statistica.software.informer.com/13.0/
EMBOSS Needle (Protein sequence analysis) Madeira et al. (2019) https://www.ebi.ac.uk/Tools/psa/emboss_needle/
Fiji version 1.49v, 1.52p, 1.53c Schindelin et al. (2012) RRID: SCR_002285;
https://imagej.net/Fiji.html#Downloads
GraphPad Prism version 7.04 and 8.0 GraphPad Software RRID: SCR_002798;
https://www.graphpad.com/scientific-software/prism/
ImageJ version 1.50b, 1.51r Schneider et al. (2012) RRID: SCR_003070;
https://imagej.nih.gov/ij/
Image Lab version 5.2.1 and 6.0.1 Bio-Rad RRID: SCR_014210;
http://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z
ImageQuant TL version 8.1 Cytiva RRID: SCR_014246;
https://us.vwr.com/store/product/25990402/imagequant-tl-8-1-cytiva-formerly-ge-healthcare-life-sciences
Image Studio Lite Version 5.2 Li-Cor RRID: SCR_013715;
https://www.licor.com/bio/image-studio-lite/download
Imaris 9.1 Bitplane AG RRID: SCR_00730; https://imaris.oxinst.com/packages
NIS Elements version 4.13.04 Nikon RRID: SCR_014329;
https://www.microscope.healthcare.nikon.com/de_EU/products/software/nis-elements
Phylogenetic analysis Bockwoldt et al. (2019) https://github.com/MolecularBioinformatics/Phylogenetic-analysis
RStudio RStudio RRID: SCR_000432; https://rstudio.com/products/rstudio/download/
Tecan i-control software version 1.10.4.0 Tecan https://lifesciences.tecan.de/plate_readers/infinite_200_pro?p=tab-3
TrackMate Tinevez et al. (2017) https://github.com/fiji/TrackMate/releases
TScratch Gebäck et al. (2009) RRID: SCR_014282;
https://github.com/cselab/TScratch
ZEN2012 blue edition Zeiss https://www.zeiss.de/mikroskopie/produkte/mikroskopsoftware/zen.html#downloads

Other

24 well plates TPP Cat# 92424
6 cm cell culture dish Greiner bio-one Cat# 628160
10 cm cell culture dish TPP Cat# 93100
15 cm cell culture dish TPP Cat# 93150
70 Ti Rotor for ultracentrifuge Beckman Coulter Cat# 337922
AxioObserver Z1 Zeiss N/A
Beckman Optima L-70K Ultracentrifuge Beckman Coulter Cat# 8043-30-1187
ChemiDoc XRS+ Bio Rad Cat# 1708265
Cover Glass VWR international Cat# 631-0130
E-plate 16 for RTCA ACEA Biosciences, Inc. Cat# 05469813001
Falcon® 24-well TC-treated Cell Polystyrene Permeable Support Companion Plate, with Lid Corning Cat# 353504
FluoroBlok 24-well Plate Permeable Support with 8.0 μm Colored PET Membrane Corning Cat# 351152
FUSION FX7 with the DarQ-9 camera Vilber N/A
ibidi culture-insert 2 well ibidi Cat# 80209
iBlot gel transfer stacks nitrocellulose membrane Thermo Fisher Scientific Cat# IB301002
LAS-4000 mini camera system GE Healthcare N/A
LAS-4000 camera system GE Healthcare N/A
Lightsheet Z.1 Zeiss N/A
LS Column Miltenyi Biotec Cat# 130-042-401
LSM 780 confocal microscope Zeiss N/A
Microscope slides Thermo Fisher Scientific Cat# 4951PLUS4
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell system Bio Rad Cat# 1658029FC
Nikon ECLIPSE Ti-E/B Nikon N/A
Odyssey 2.1 imaging system Li-Cor, USA N/A
QuadroMACS Separator Miltenyi Biotec Cat# 130-090-976
RTCA Control Unit with RTCA Software ACEA Biosciences, Inc. Cat# 05454417001
RTCA DP Analyzer ACEA Biosciences, Inc. Cat# 05469759001
Tecan Infinite 200 PRO plate reader Tecan N/A
ZebraboxTM ViewPoint N/A