REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
CALR | Cell Signaling | Cat# 12238; RRID: AB_2688013 |
CANX | Cell Signaling | Cat# 2679; RRID: AB_2228381 |
CTSD | Cell Signaling | Cat# 2284; RRID: AB_10694258 |
EEA1 | Cell Signaling | Cat# 3288; RRID: AB_2096811 |
EIF2S1 | Cell Signaling | Cat# 9722; RRID: AB_2230924 |
EIF2S1-pS51 | Cell Signaling | Cat# 9721; RRID: AB_330951 |
EIF3A | Cell Signaling | Cat# 3411; RRID: AB_2096523 |
FLAG | Sigma-Aldrich | Cat# F3165; RRID: AB_259529 |
G3BP1 | Santa Cruz | Cat# sc-365338; RRID: AB_10846950 |
G3BP1 | Santa Cruz | Cat# sc-81940; RRID: AB_1123055 |
G3BP2 | Bethyl | Cat# A302-040A; RRID: AB_1576545 |
GAPDH | Abcam | Cat# ab37187; RRID: AB_732651 |
GAPDH (zebrafish) | Sigma-Aldrich | Cat# SAB2701826 |
GFP | Roche | Cat# 11814460001; RRID: AB_390913 |
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Invitrogen | Cat# A-11001; RRID: AB_2534069 |
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | Invitrogen | Cat# A-11011; RRID: AB_143157 |
Goat anti-Mouse IgG (H+L) cross-adsorbed secondary, Alexa Fluor 555 | Thermo Fisher Scientific | Cat# A-21422; RRID: AB_2535844 |
Goat anti-Rabbit IgG (H+L) cross-adsorbed secondary, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11008; RRID: AB_143165 |
Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP-coupled | Thermo Fisher Scientific | Cat# 31430; RRID: AB_228307 |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP-coupled | Thermo Fisher Scientific | Cat# 31460; RRID: AB_228341 |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Dylight 800 (zebrafish) | Thermo Fisher Scientific | Cat# SA5-35571; RRID: AB_2556775 |
Goat anti-Rat IgG (H+L) Secondary Antibody, HRP-coupled | Thermo Fisher Scientific | Cat# 31470; RRID: AB_228356 |
GOLGA1 | Cell Signaling | Cat# 13192; RRID: AB_2798144 |
GOLGA2 | Cell Signaling | Cat# 12480; RRID: AB_2797933 |
HA | Roche | Cat# 11867423001; RRID: AB_390918 |
Histone H3 (H3C1) | Bethyl | Cat# A300-822A; RRID: AB_597872 |
HSP60 (HSPD1) | Cell Signaling | Cat# 12165; RRID: AB_2636980 |
HSP90 (CDC37) | Cell Signaling | Cat# 4877; RRID: AB_2233307 |
LMNA A/C | Cell Signaling | Cat# 2032; RRID: AB_2136278 |
LAMP1 | Cell Signaling | Cat# 9091; RRID: AB_2687579 |
LAMP1 | Developmental Studies Hybridoma Bank | Cat# H4A3; RRID: AB_2296838 |
LAMP2 | Cell Signaling | Cat# 49067;RRID: AB_2799349 |
LAMP2 | Santa Cruz | Cat# sc-18822; RRID: AB_626858 |
LAMP2 | Developmental Studies Hybridoma Bank | Cat# H4B4; RRID: AB_2134755 |
MTOR | Cell Signaling | Cat# 2983; RRID: AB_2105622 |
MTOR epitope maps to residues 221 and 261 of human mTOR | Monoclonal Antibody Core Unit. Helmholtz Center Munich, Germany |
TQREP-3G6 |
Mock antibody mouse | Santa Cruz | Cat# sc-2025; RRID: AB_737182 |
Mock antibody rabbit | Bethyl | Cat# P120-101; RRID: AB_479829 |
Sigma-Aldrich | Cat# I5006; RRID: AB_1163659 | |
Mock antibody rat | Monoclonal Antibody Core Unit. Helmholtz Center Munich, Germany |
RmC3-7H8 |
Mouse IgG HRP Linked Whole Ab | Merck | Cat# GENA931 |
MYC-tag | Cell Signaling | Cat# 2276; RRID: AB_331783 |
Rabbit IgG HRP Linked Whole Ab | Merck | Cat# GENA934; RRID: AB_2722659 |
RPS6KB1 | Cell Signaling | Cat# 2708; RRID: AB_390722 |
RPS6KB1-pT389 | Cell Signaling | Cat# 9206; RRID: AB_2285392 |
RPS6KB1-pT389 | Cell Signaling | Cat# 9205; RRID: AB_330944 |
RAB5A | Cell Signaling | Cat# 3547; RRID: AB_2300649 |
RAB7A | Cell Signaling | Cat# 9367; RRID: AB_1904103 |
RPTOR | Cell Signaling | Cat# 2280; RRID: AB_561245 |
RPTOR #1 epitope maps to residues 686 and 704 of human Raptor | Monoclonal Antibody Core Unit. Helmholtz Center Munich, Germany |
RAP1-20C4 |
RPTOR #2 | Bethyl | Cat# A300-553A; RRID: AB_2130793 |
RPS6 | Cell Signaling | Cat# 2317; RRID: AB_2238583 |
RPS6-pS235/236 | Cell Signaling | Cat# 4856; RRID: AB_2181037 |
RPS6-pS235/236 (zebrafish) | Cell Signaling | Cat# 2211; RRID: AB_331679 |
RPS6-pS235/236 (zebrafish) | Cell Signaling | Cat# 4858; RRID: AB_916156 |
TSC1 | Cell Signaling | Cat# 4906; RRID: AB_2209790 |
TSC1 #1 | Gift from Michael N. Hall, Basel, Switzerland (Molle, 2006). Generated according to van Slegtenhorst et al. (1998). | N/A |
TSC1 #2 | Thermo Fisher Scientific (Invitrogen) | Cat# 37-0400; RRID: AB_2533292 |
TSC1 #3 | Cell Signaling | Cat# 6935; RRID: AB_10860420 |
TSC2 | Cell Signaling | Cat# 4308; RRID: AB_10547134 |
TSC2 #1 | Thermo Fisher Scientific (Invitrogen) | Cat# 37-0500; RRID: AB_2533293 |
TSC2 #2 epitope maps to residues 1535 and 1784 of human TSC2 | Gift from Michael N. Hall, Basel, Switzerland (Molle, 2006). Generated according to van Slegtenhorst et al. (1998). | N/A |
TSC2 #3 | Abcam | Cat# ab52936; RRID: AB_883283 |
TUBA1B | Abcam | Cat# ab108629; RRID: AB_10866252 |
VDAC | Cell Signaling | Cat# 4661; RRID: AB_10557420 |
For antibody dilutions, see Table S3 | ||
Bacterial and Virus Strains | ||
DB3.1 | Thermo Fisher Scientific | Cat# 11782018 (discontinued) |
DH5-alpha | New England Biolabs | Cat# C2987H |
Chemicals, Peptides, and Recombinant Proteins | ||
AEBSF (4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride) | Sigma-Aldrich | Cat# A8456 |
Aprotinin | Sigma-Aldrich | Cat# A1153 |
Benzamidine | Sigma-Aldrich | Cat# B6506 |
Beta-Mercaptoethanol | GIBCO | Cat# 21-985-023 |
Bromophenol Blue | Sigma-Aldrich | Cat# B5525 |
BSA (bovine serum albumin) | Carl Roth | Cat# 8076.5 |
Calcein AM Fluorescent Dye | Corning | Cat# 354216 |
CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate) | Sigma-Aldrich | Cat# 3023 |
CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate) (for IPs in rat brain tissue) |
Roth | Cat# 1479.3 |
Complete Protease Inhibitor Cocktail | Sigma-Aldrich | Cat# D27802 |
DABCO (1,4-diazabicyclo[2.2.2]octane) | Merck | Cat# 11836153001 |
DexoMAG® | Liquids Research | N/A |
DMEM (Dulbecco’s Modified Eagle’s Medium) w: 4.5 g/L Glucose, w/o: L-Glutamine, w: Sodium pyruvate, w: 3.7 g/L NaHCO3 | PAN | Cat# P04-03600 |
DMEM (Dulbecco’s Modified Eagle’s Medium) used for PLA experiments in Figure 4A | Thermo Fisher Scientific | Cat# 41965-039 |
DMEM (Dulbecco’s Modified Eagle’s Medium) used for lysosomal isolations and trypsin digests | Thermo Fisher Scientific | Cat# 41965-062 |
DMSO (dimethyl sulfoxide) | Sigma-Aldrich | Cat# D2650 |
Doxycycline | Sigma-Aldrich | Cat# D3447 |
Duolink In Situ Mounting Medium with DAPI | Sigma-Aldrich | Cat# DUO82040 |
Dynabeads Protein G for Immunoprecipitation | Thermo Fisher Scientific | Cat# 10009D |
E64 | Sigma-Aldrich | Cat# E3132 |
Ethosuximide | Sigma-Aldrich | Cat# 1001096708 |
FBS (fetal bovine serum) | GIBCO | Cat# 10270106 |
FBS (fetal bovine serum) | Sigma-Aldrich | Cat# F9665 |
Glycerol | Sigma-Aldrich | Cat# G5516 |
Glycine | Sigma-Aldrich | Cat# G7126 |
HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) | Life technologies | Cat# 15630080 |
HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) | Life technologies | Cat# 15630106 |
HBSS (Hank’s Balanced Salt Solution) w/o: Phenol red, w: Ca and Mg, w: 0.35 g/L NaHCO3 | PAN | Cat# P04-32505 |
Hoechst 33342 (dilution in IF: 1:100,000) | Invitrogen | Cat# H3570 |
Insulin | Sigma-Aldrich | Cat# I1882 |
IGEPAL CA-630 (NP40) | Sigma-Aldrich | Cat# I8896 |
Imidazole | Sigma-Aldrich | Cat# I0250 |
KCl (potassium chloride) | Sigma-Aldrich | Cat# P9541 |
Leupeptin | Sigma-Aldrich | Cat# 103476-89-7 |
L-glutamine | GIBCO | Cat# 25030024 |
L-glutamine | GIBCO | Cat# 25030081 |
Methanol | Klinipath | Cat# 4063-9005 |
MgCl2 (magnesium chloride) | Sigma-Aldrich | Cat# M2670 |
MK2206 | Axon Medchem | Cat# 1684 |
Mowiol 4-88 | Carl Roth | Cat# 07131 |
NaCl (sodium chloride) | Sigma-Aldrich | Cat# S7653 |
NPG (n-propyl-gallate) | VWR | Cat# EM8.20599.0500 |
PBS (phosphate-buffered saline) | PAN | Cat# P04-36500 |
PBS (phosphate-buffered saline) for non-sterile washing | Biochrom | Cat# L182-50 |
Penicillin/Streptomycin | GIBCO | Cat# 15140122 |
Pepstatin A | Sigma-Aldrich | Cat# 26305-03-3 |
PhosSTOP for IPs in brain tissue | Merck | Cat# 4906837001 |
Phosphatase Inhibitor Cocktail 2 | Sigma-Aldrich | Cat# P5726 |
Phosphatase Inhibitor Cocktail 3 | Sigma-Aldrich | Cat# P0044 |
Phusion HF DNA Polymerase | Roche | Cat# M0530S |
Pierce 16% Formaldehyde (w/v), Methanol-free | Thermo Fisher Scientific | Cat# 28908 |
Polyacrylamide | Bio-Rad | Cat# 161-0159 |
Polybrene | Sigma-Aldrich | Cat# H9268 |
Prolong Gold antifade reagent with 4′,6-Diamidin-2-phenylindol (DAPI) | Thermo Fisher Scientific | Cat# P36935 |
Protein G Sepharose beads | GE | Cat# 17061801 |
Puromycin | Sigma-Aldrich | Cat# P8833 |
PVDF (polyvinylidene difluoride) membrane | Millipore | Cat# IPVH00010 |
Rapamycin | Calbiochem | Cat# 553210 |
SDS (sodium dodecyl sulfate) | Sigma-Aldrich | Cat# 71725 |
Sodium deoxycholate | Sigma-Aldrich | Cat# 30970 |
Sodium fluoride | Sigma-Aldrich | Cat# 7681-49-4 |
Sodium glycerophosphate | Sigma-Aldrich | Cat# G9322 |
Sodium orthovanadate | Sigma-Aldrich | Cat# 13721-39-6 |
Sodium pyrophosphate | Sigma-Aldrich | Cat# 13472-36-1 |
Sucrose | Sigma-Aldrich | Cat# S2395 |
T4 DNA Ligase | New England Biolabs | Cat# M0202S |
Torin1 | Axon Medchem | Cat# 1833 |
Transfectin | Biorad | Cat# 1703350 |
Tricaine (MS-222) | Sigma-Aldrich | Cat# E10521 |
TRIS base (tris(hydroxymethyl)aminomethane) | VWR | Cat# A1086.5000 |
Triton X-100 | Sigma-Aldrich | Cat# 93443 |
Trypan Blue | GIBCO | Cat# 15250061 |
Trypsin | GIBCO | Cat# 15400054 |
Tween-20 | MP Biomedicals | Cat# 11TWEEN201 |
Critical Commercial Assays | ||
Bio-Rad Protein Assay Dye Reagent Concentrate | Bio-Rad | Cat# 500-0006 |
Duolink In Situ Red Starter Kit Mouse/Rabbit | Sigma-Aldrich | Cat# DUO92008 |
Duolink® In Situ PLA® Probe Anti-Rabbit PLUS Affinity purified Donkey anti-Rabbit IgG (H+L) | Sigma-Aldrich | Cat# DUO92002; RRID: AB281940 |
Duolink® In Situ PLA® Probe Anti-Mouse MINUS Affinity purified Donkey anti-Mouse IgG (H+L) | Sigma-Aldrich | Cat# DUO92004; RRID: AB_2713942 |
JetPEI | Poly-Plus | Cat# 101-40N |
Lipofectamine 3000 Transfection Reagent | Thermo Fisher Scientific | Cat# L3000015 |
Lipofectamine RNAiMAX Transfection Reagent | Thermo Fisher Scientific | Cat# 13778150 |
MidiPrepKit NUCLEOBOND XTRA MIDI | Macherey-Nagel | Cat# 740410.50 |
NuPage MES SDS running buffer | Thermo Fisher Scientific | Cat# NP0002 |
NuPage Novex 10% Bis-Tris gel | Thermo Fisher Scientific | Cat# NP0302BOX |
Odyssey blocking buffer | Li-Cor | Cat# 927-40000 |
Pierce BCA protein assay kit | Thermo Fisher Scientific | Cat# 23225 |
Pierce ECL Western Blotting Substrate | Thermo Fisher Scientific | Cat# 32209 |
RIPA buffer (zebrafish lysis) | Merck | Cat# R0278 |
SuperSignal West FEMTO Maximum Sensitivity Substrate | Thermo Fisher Scientific | Cat# 34095 |
Trans-Lentiviral shRNA Packaging Mix | Dharmacon | Cat# TLP5912 |
Deposited Data | ||
Invasive breast cancer (The Cancer Genome Atlas, TCGA, provisional) | http://www.cbioportal.org | RRID: SCR_014555 |
TSC1 RNA expression data | http://www.kmplot.com | probeID: 209390_at |
TSC2 RNA expression data | http://www.kmplot.com | probeID: 215735_s_at |
G3BP1 RNA expression data | http://www.kmplot.com | probeID: 225007_at |
G3BP1 protein expression data | http://www.kmplot.com | probeID: Q13283 |
Experimental Models: Cell Lines | ||
HEK293T | Thien et al. (2015) | N/A |
HEK293T Control | This paper | N/A |
HEK293T G3BP1 KO | This paper | N/A |
HEK293-β2AR | Lavoie et al. (2002) | N/A |
HeLa alpha Kyoto | Thedieck et al. (2007) | N/A |
HeLa S3 | ATCC | Cat# CCL-2.2; RRID: CVCL_0058 |
MCF-7 ACC115 | DSMZ | Cat# ACC115; RRID: CVCL_0031 |
MCF-7 Control (for G3BP1) | This paper | N/A |
MCF-7 Control (for TSC2) | This paper | N/A |
MCF-7 G3BP1 KO | This paper | N/A |
MCF-7 GFP-LC3 | Gift from Joern Dengjel, Fribourg, Switzerland | N/A |
MCF-7 shControl | This paper | N/A |
MCF-7 shG3BP1 #1 | This paper | N/A |
MCF-7 shG3BP1 #2 | This paper | N/A |
MCF-7 TSC2 KO | This paper | N/A |
MDA-MB-231 | ATCC | Cat# HTB-26; RRID: CVCL_0062 |
MDA-MB-231 TSC Control | This paper | N/A |
MDA-MB-231 TSC2 KO | This paper | N/A |
MDA-MB-231 shControl | This paper | N/A |
MDA-MB-231 shG3BP1 #1 | This paper | N/A |
MDA-MB-231 shG3BP1 #2 | This paper | N/A |
Experimental Models: Organisms/Strains | ||
AB Danio rerio | Zebrafish International Resource Center | Cat# ZL1; RRID: ZIRC_ZL1 |
Tg(dlx5a/dlx6a-EGFP) x Tg(vglut2a:loxP-RFP-loxP-GFP) |
Noble et al. (2015)Tg(dlx5a/dlx6a-EGFP); Satou et al. (2012)Tg(vglut2a:loxP-RFP-loxP-GFP) |
N/A |
Tg(HuC:GCaMP5G) | Ahrens et al. (2013) | N/A |
Wistar Cmd:(WI)WU rats | Mossakowski Medical Research Centre Polish Academy of Sciences | N/A |
Oligonucleotides | ||
control MO: 5′-CCTCTTACCTCAGTTACAATTTATA-3′ | GeneTools | N/A |
g3bp1 MO: 5′-TAACAAAGGGCAAGTCACCTGTGCA-3′ | GeneTools | N/A |
tsc2 MO: 5′-CTGTGTGTTTTCTTACTGGACCATA-3′ | GeneTools | N/A |
For primers, see Table S4 | N/A | |
For RNA, see Table S5 | N/A | |
Recombinant DNA | ||
bFos-MYC-LC151 | Gift from Qingming Luo, Wuhan, China (Chu et al., 2009) | N/A |
bJun-HA-LN151 | Gift from Qingming Luo, Wuhan, China (Chu et al., 2009) | N/A |
lentiGuide-Puro | Sanjana et al. (2014) | RRID: Addgene_52963 |
pCMV6-AN-MYC-DDK (MYC-FLAG-empty) | Origene | Cat# PS100016 |
MYC-FLAG-G3BP1 | This paper | N/A |
MYC-FLAG-G3BP1-ΔRGG (aa 410-466) | This paper | N/A |
MYC-FLAG-G3BP1-ΔNTF2L (aa 1-139) | This paper | N/A |
MYC-FLAG-G3BP1-NTF2L 1-139 | This paper | N/A |
MYC-FLAG-G3BP2 | This paper | N/A |
pCW-Cas9-Blast | Sanjana et al. (2014) | RRID: Addgene_83481 |
pGW-MYC-LC151 | Stefan Pusch (Weiler et al., 2014) | N/A |
pGW-HA-LN151 | Stefan Pusch (Weiler et al., 2014) | N/A |
pGW-HA-LN151-G3BP1 | This paper | N/A |
pGW-MYC-LC151-G3BP1 | This paper | N/A |
pGW-MYC-LC151-G3BP1 1-182 | This paper | N/A |
pGW-MYC-LC151-G3BP1 183-332 | This paper | N/A |
pGW-MYC-LC151-G3BP1 333-466 | This paper | N/A |
pGW-HA-LN151-G3BP2 | This paper | N/A |
pGW-MYC-LC151-G3BP2 | This paper | N/A |
pGW-HA-LN151-LAMP1 | This paper | N/A |
pGW-MYC-LC151-LAMP1 | This paper | N/A |
pGW-MYC-LC151-LAMP1 1-382 | This paper | N/A |
pGW-MYC-LC151-LAMP1 383-417 | This paper | N/A |
pGW-HA-LN151-LAMP2 | This paper | N/A |
pGW-HA-LN151-mTOR | This paper | N/A |
pGW-HA-LN151-TSC2 | This paper | N/A |
pEGFP-C-LAMP2 | This paper | N/A |
pEGFP-C-TSC2 | This paper | N/A |
pEGFP-C (derivate of pDEST with a C-terminal EGFP tag) | Stefan Pusch | N/A |
pDEST | Stefan Pusch; Clone repository of the DKFZ Genomics and Proteomics Core Facility (GPCF) |
N/A |
pDONR201-tGFP | This paper | N/A |
pDONR201-tGFP-LAMP1 383-417 | This paper | N/A |
pENTR221-G3BP1 | Clone repository of the DKFZ Genomics and Proteomics Core Facility (GPCF) |
CloneId: 182373397 |
pENTR223-G3BP2 | Clone repository of the DKFZ Genomics and Proteomics Core Facility (GPCF) |
CloneId: 192451551 |
pENTR221-LAMP1 | Clone repository of the DKFZ Genomics and Proteomics Core Facility (GPCF) |
CloneId: 193137117 |
pENTR221-LAMP2 | Clone repository of the DKFZ Genomics and Proteomics Core Facility (GPCF) |
CloneId: 115072391 |
pSpCas9(BB)-2A-Puro (PX459) V2.0 | Addgene | RRID: Addgene_62988 |
psPAX2 | Shalem et al. (2014) | RRID: Addgene_12260 |
pMD2.G | Shalem et al. (2014) | RRID: Addgene_12259 |
R777-E138 Hs.MTOR-nostop | Gift from Dominic Esposito, Addgene | Cat# 70422; RRID: Addgene_70422 |
R777-E356 Hs.TSC2-nostop | Gift from Dominic Esposito, Addgene | Cat# 70640; RRID: Addgene_70640 |
Software and Algorithms | ||
Adobe Photoshop version CS5.1 | Adobe Systems Incorporated | RRID: SCR_014199;https://www.adobe.com/de/products/photoshop.html# |
Bash script to identify the single nucleotide variants (SNV) within G3BP1 and G3BP2 | This paper | Document S3 |
Cell Profiler version 3.1.5 | McQuin et al. (2018) | RRID: SCR_007358; https://www.cellprofiler.org/ |
CGDS-R package version 1.2.6 | Gao et al. (2013) | https://github.com/cBioPortal/cgdsr |
Dell Statistica version 13 | Dell Inc. | https://statistica.software.informer.com/13.0/ |
EMBOSS Needle (Protein sequence analysis) | Madeira et al. (2019) | https://www.ebi.ac.uk/Tools/psa/emboss_needle/ |
Fiji version 1.49v, 1.52p, 1.53c | Schindelin et al. (2012) | RRID: SCR_002285; https://imagej.net/Fiji.html#Downloads |
GraphPad Prism version 7.04 and 8.0 | GraphPad Software | RRID: SCR_002798; https://www.graphpad.com/scientific-software/prism/ |
ImageJ version 1.50b, 1.51r | Schneider et al. (2012) | RRID: SCR_003070; https://imagej.nih.gov/ij/ |
Image Lab version 5.2.1 and 6.0.1 | Bio-Rad | RRID: SCR_014210; http://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z |
ImageQuant TL version 8.1 | Cytiva | RRID: SCR_014246; https://us.vwr.com/store/product/25990402/imagequant-tl-8-1-cytiva-formerly-ge-healthcare-life-sciences |
Image Studio Lite Version 5.2 | Li-Cor | RRID: SCR_013715; https://www.licor.com/bio/image-studio-lite/download |
Imaris 9.1 | Bitplane AG | RRID: SCR_00730; https://imaris.oxinst.com/packages |
NIS Elements version 4.13.04 | Nikon | RRID: SCR_014329; https://www.microscope.healthcare.nikon.com/de_EU/products/software/nis-elements |
Phylogenetic analysis | Bockwoldt et al. (2019) | https://github.com/MolecularBioinformatics/Phylogenetic-analysis |
RStudio | RStudio | RRID: SCR_000432; https://rstudio.com/products/rstudio/download/ |
Tecan i-control software version 1.10.4.0 | Tecan | https://lifesciences.tecan.de/plate_readers/infinite_200_pro?p=tab-3 |
TrackMate | Tinevez et al. (2017) | https://github.com/fiji/TrackMate/releases |
TScratch | Gebäck et al. (2009) | RRID: SCR_014282; https://github.com/cselab/TScratch |
ZEN2012 blue edition | Zeiss | https://www.zeiss.de/mikroskopie/produkte/mikroskopsoftware/zen.html#downloads |
Other | ||
24 well plates | TPP | Cat# 92424 |
6 cm cell culture dish | Greiner bio-one | Cat# 628160 |
10 cm cell culture dish | TPP | Cat# 93100 |
15 cm cell culture dish | TPP | Cat# 93150 |
70 Ti Rotor for ultracentrifuge | Beckman Coulter | Cat# 337922 |
AxioObserver Z1 | Zeiss | N/A |
Beckman Optima L-70K Ultracentrifuge | Beckman Coulter | Cat# 8043-30-1187 |
ChemiDoc XRS+ | Bio Rad | Cat# 1708265 |
Cover Glass | VWR international | Cat# 631-0130 |
E-plate 16 for RTCA | ACEA Biosciences, Inc. | Cat# 05469813001 |
Falcon® 24-well TC-treated Cell Polystyrene Permeable Support Companion Plate, with Lid | Corning | Cat# 353504 |
FluoroBlok 24-well Plate Permeable Support with 8.0 μm Colored PET Membrane | Corning | Cat# 351152 |
FUSION FX7 with the DarQ-9 camera | Vilber | N/A |
ibidi culture-insert 2 well | ibidi | Cat# 80209 |
iBlot gel transfer stacks nitrocellulose membrane | Thermo Fisher Scientific | Cat# IB301002 |
LAS-4000 mini camera system | GE Healthcare | N/A |
LAS-4000 camera system | GE Healthcare | N/A |
Lightsheet Z.1 | Zeiss | N/A |
LS Column | Miltenyi Biotec | Cat# 130-042-401 |
LSM 780 confocal microscope | Zeiss | N/A |
Microscope slides | Thermo Fisher Scientific | Cat# 4951PLUS4 |
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell system | Bio Rad | Cat# 1658029FC |
Nikon ECLIPSE Ti-E/B | Nikon | N/A |
Odyssey 2.1 imaging system | Li-Cor, USA | N/A |
QuadroMACS Separator | Miltenyi Biotec | Cat# 130-090-976 |
RTCA Control Unit with RTCA Software | ACEA Biosciences, Inc. | Cat# 05454417001 |
RTCA DP Analyzer | ACEA Biosciences, Inc. | Cat# 05469759001 |
Tecan Infinite 200 PRO plate reader | Tecan | N/A |
ZebraboxTM | ViewPoint | N/A |