Figure 3.

miR-19a/b directly targeted SEPT7 in GBM cells

(A) Venn diagram displaying miR-19a/b computationally predicted to target SEPT7 by three different prediction algorithms: TargetScan, miRDB, and microT-CDS. (B) Predicted miR-19a/b binding sequence in 3′ UTR of SEPT7 as shown by HuMiTar. (C) Luciferase assay of cells transfected with SEPT7 3′ UTR luciferase reporter plasmid together with miR-19a/b inhibitors or miR-NC inhibitor. (D) SEPT7 expression in glioma cells transfected with miR-19a/b inhibitors and miR-19a/b mimics was detected by western blotting . GAPDH was used as the loading control. ∗∗p < 0.01.