Figure 5.

Regulation of miR-223-3p upon TNF-α-induced insulin resistance in adipocytes

(A–C and H–J) Extracellular (A and H) and intracellular (B and I) levels of miR-223-3p measured in the culture media and in the cell extracts, respectively, and miR-223-3p extracellular/intracellular ratio in control or TNF-α-treated 3T3-L1 preadipocytes and adipocytes, respectively (C and J). (D–N) mRNA expression of Ago2 (D and K), Cd63 (E and L), Hnrnpa2b1 (F and M), and Ybx1 (G and N) in control or TNF-α-treated 3T3-L1 preadipocytes (D−G) or adipocytes (K−N). (O) Concentration of exosomes in cell culture supernatant from 3T3-L1 cells upon 24 h of treatment with 5 nM TNF-α or vehicle (control). (P–R) Extracellular (P) and intracellular (Q) levels of miR-223-3p measured in the culture media and in the cell extracts, respectively, and miR-223-3p extracellular/intracellular ratio (R) in control or TNF-α-treated THP-1 cells. Values are given as mean ± SEM (n = 6), ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus control. See also Figures S1−S3.