Figure 3.

The cytokine profile of effector cells was characterized following 20-h exposure to target cells at an E:T ratio of 5:1

(A) Pro-inflammatory, (B) Regulatory, (C) Anti-inflammatory and (D) Chemotactic factors produced by effector cells following co-culture were quantitated. Effector cells are T cells (no CAR) (blue) or TAG-72.CD28 CAR (red) or TAG-72.4-1BB CAR (orange) with the target cell lines OVCAR-3 and MESOV, respectively. Biological and intra-assay triplicates were used and are denoted as average ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.