Figure 6.

In vitro, dual-specific CARs demonstrate enhanced ability to eliminate cancer cells expressing low levels of the target antigen

(A–D) Mono-specific TAG-72 CAR-T cells and dual-specific TAG-72 CAR + ΔCD47 CAR-T cells were co-cultured with either OVCAR-3 (A) or MESOV (B) cancer cell lines or HLF (C) or HUF (D) non-cancerous fibroblast cell lines at an E:T ratio of 5:1. Target cell response kinetics were measured in real time using xCELLigence (left panels) where data represent the average normalized cell index of 4–11 biological replicates ± SEM. Efficiency of target cell killing was quantitated at 10 and 20 h of co-culture and is presented as average percent cytotoxicity ± SD (right panels). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001.