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. 2017 Sep 20;52(6):629–637. doi: 10.1093/alcalc/agx063

Fig. 4.

Fig. 4.

Cytochrome P450 2E1 (CYP 2E1) is induced in cHAP but not C57BL/6 (C57) EtOH-DW+binge mice. (A) Representative immunoblots CYP2E1 protein expression in hepatic tissue lysates from C57BL/6 and cHAP mice maintained on either Lieber-DeCarli control liquid diet + maltose-dextrin (LDC+MD), Lieber-DeCarli ethanol liquid diet+binge (LDE+B) or free access 10% (v/v) ethanol in DW + binge (EtOH-DW+B). Densitometric analysis was performed and fold change in expression was determined and corrected using Ponceau S staining of membranes performed following transfer prior to antibody detection. #P < 0.05 strain-matched LDE+B or EtOH-DW+B versus LDC+MD. (B) CYP2E1 activity in tissue lysates expressed as pMol 4-nitrocatechol formation per mg tissue per minute. #P < 0.05 strain-matched LDE+B or EtOH-DW+B versus LDC+MD.