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. 2020 Sep 6;19(2):240–250. doi: 10.1111/pbi.13456

Figure 4.

Figure 4

Small‐scale SV in a key flowering‐time gene Vernalization Insensitive 3 on chromosome C03 (BnVIN3.C03). (a) 90 bp insertion detected only in accession Express 617 (highlighted in the black box) in an orthologue BnVIN3.C03 was revealed by aligning ONT reads from 4 different genotypes to the Darmor‐bzh reference version 4.1. (b) Agarose gel image of PCR products spanning the insertion polymorphism. M:100 bp ladder; 1: N99; 2: PAK85912; 3: Express 617; 4: R53. As expected, Express 617 exhibits 1170 bp PCR product, whereas the other three genotypes all show a 1060 bp amplicon.