Celastrol reduces the BW of ob/ob mice fed an HFD
(A–J) HFD-fed obese ob/ob mice were subjected to oral administration of celastrol (55.2 ppm) for 6 weeks. (A) Representative pictures of male obese ob/ob mice after 6 weeks of celastrol treatment. (B) BW of ob/ob mice during the treatment (n = 6 for each group). (C) The average 3-day food intake of ob/ob mice during celastrol treatment (n = 6 for each group). (D) Macroscopic view of representative sections of eWAT, iWAT, pWAT, and BAT of ob/ob mice treated with celastrol or not. (E–H) Weights of (E) eWAT, (F) iWAT, (G) pWAT, and (H) BAT of ob/ob mice treated with celastrol or not (n = 6 for each group). (I and J) Results for (I) GTT and (J) the AUC for GTTs (n = 6 for each group). Error bars represent the mean ± SEM. p values were determined by two-way ANOVA (B) or Student's t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; n.s., not significant (p > 0.05).