Early intervention with celastrol inhibits intestinal lipid absorption and prevents HFD-induced weight gain in C57BL/6 mice
(A–K) ND- or HFD-fed C57BL/6 mice were subjected to oral administration of celastrol (3 mg/kg/day) for 24 days. (A) The BW of each group of mice during the treatment (n = 10 for each group). (B) Macroscopic view of representative sections of eWAT, iWAT, pWAT, and BAT of each group of mice. (C–F) Weights of (C) eWAT, (D) iWAT, (E) pWAT, and (F) BAT of each group of mice (n = 10 for each group). (G) The average 3-day food intake of each group of mice during celastrol treatment (n = 10 for each group). (H) Relative fecal TG levels of each group of mice during celastrol treatment (n = 10 for each group). (I–J) Representative pictures of isolated intestines of celastrol-treated mice (I) on an ND or (J) HFD. (K) Representative H&E-stained images of the intestine (200×). Scale bar, 50 μm. Error bars represented the mean ± SEM. p values were determined by two-way ANOVA. ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05; n.s., not significant (p > 0.05).