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. 2021 Jan 20;23:930–943. doi: 10.1016/j.omtn.2021.01.016

Figure 6.

Figure 6

miR-22-3p inhibits PI3K/Akt and Src signaling by suppressing eEF2K expression

(A) Western blot analysis showing that ectopic expression of miR-22-3p inhibited the expression of eEF2K, p-Akt (Ser473), p-Src (Tyr416), and p-EF2 (Thr56) in cells treated with miR-22-3p. TNBC cells were analyzed following transfection with a miR-22-3p mimic (72 h) or control miRNA. (B) Western blot analysis of p-Akt (Ser473), p-Src (Tyr416), and p-EF2 (Thr56) protein levels in MDA-MB-231 cells after siRNA-mediated knockdown of eEF2K. (C and D) Effect of miR-22-3p transfection in eEF2K-overexpressed MDA-MB-231 cells on cell proliferation and colony formation (10 days). ∗p < 0.05; n.s, not significant.