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. 2021 Jan 30;22(3):1389. doi: 10.3390/ijms22031389

Figure 1.

Figure 1

Neonatal 3% sevoflurane exposure to postnatal day 7 (PD7) mice for 6 h induces acute apoptosis in hippocampi and results in impaired memory of PD60 adult mice. (A) Caspase 3 activity assay shows an increase in neuroapoptosis in the PD7 mouse hippocampi immediately after exposure to 3% sevoflurane for 6 h. Data were presented with mean ± SD (n = 4 per group; *: p < 0.05. (B) Fluorescence images of the immunofluorescence-stained brain sagittal sections demonstrate that sevoflurane treatment increased activated caspase 3-positive cells (green) in the PD7 mouse hippocampi. Scale bar: 100 μm. Blue represents Hoechst 33342-stained cell nuclei. (C) Morris water maze showed that neonatal sevoflurane exposure impaired memory capacity but not learning ability in PD60 mice (n = 12 for control and n = 17 for sevoflurane group); **: p < 0.01.