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. 2021 Jan 14;11:579330. doi: 10.3389/fphar.2020.579330

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Copyright © 2021 Suresh, Parida, Minz, Sethi, Sahoo and Senapati.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of MA5-32307 and AF933 antibodies’ reactivity and specificity toward hamster ACE2. (A) Immunoblot analysis showing the expression status of ACE2 with two different antibodies (MA5-32307 and AF933) in multiple organ tissues of the Syrian golden hamster. β-actin was used as an internal control. (B) Immunoblots showing ACE2 expression in hamster kidney, human colorectal adenocarcinoma cells (Caco2), and recombinant human ACE-2 protein (rHu-ACE2) with two different antibodies. (C) Graph showing Ace2 relative mRNA expression in hamster kidney and spleen tissues. (D) Micrographs showing immunofluorescence and immunohistochemical staining of hamster kidney with antibody alone or antibody preincubated with rHu-ACE2 protein (in IHC, scale bar = 25 µm).