Table 3.
Primary and secondary antibodies used in immunostaining
| Antibody type | Target | Species | Concentration | Source |
|---|---|---|---|---|
| Primary antibodies | Anti-CB1 | Guinea pig | 1:2000 | Fukudome et al. (2004) |
| Anti-NECAB1 | Rabbit | 1:300 | Atlas Antibodies, HPA023629 | |
| Anti-NECAB2 | Rabbit | 1:500 | Atlas Antibodies, HPA013998 | |
| Anti-CCK | Mouse | 1:3000 | CURE, 39161 | |
| Anti-GAD67 | Mouse | 1:2000 | Merck, MAB5406 | |
| Anti-PV | Mouse | 1:5000 | Swant, 235 | |
| Secondary antibodies | Anti-guinea pig, Alexa 488-conjugated | Donkey | 1:400 | Jackson, 706-545-148 |
| Anti-rabbit Alexa 594-conjugated | Donkey | 1:400 | Jackson, 711-585-152 | |
| Anti-mouse Alexa 488-conjugated | Donkey | 1:400 | Jackson, 715-545-150 | |
| Anti-guinea pig Alexa-594-conjugated | Donkey | 1:400 | Jackson, 706-585-148 | |
| Anti-rabbit Alexa 647-conjugated | Donkey | 1:400 | Jackson, 711-605-152 | |
| Anti-rabbit Alexa 488-conjugated | Donkey | 1:400 | Jackson, 711-545-152 | |
| Anti-mouse Alexa 594-conjugated | Donkey | 1:400 | Jackson, 715-585-150 | |
| Anti-mouse Alexa 647-conjugated | Donkey | 1:400 | Jackson, 715-605-150 |
Note: Referred in Materials and Methods/Immunostaining section.