Varied types of hippocampal boutons and their postsynaptic targets in A25. (A) Example of hippocampal bouton making a round synapse (arrow) on a spine (Sp). (B) Example of hippocampal bouton with mitochondria (double headed arrow) making a perforated synapse (arrow) on a spine (Sp); *: spine apparatus. (C) Example of hippocampal bouton that formed a synapse on a dendritic shaft. (D–F) Examples of hippocampal axon boutons that formed synapses on CB or CR positive elements. D, hippocampal bouton forming a synapse on CB positive dendritic shaft (double-headed arrow shows CB label in dendrite, Den); E, hippocampal bouton forming a synapse on a CB positive spine; F, hippocampal bouton forming a synapse on CR positive dendritic shaft. Black arrows show the synaptic sites. Double-headed arrows indicate the gold labeling for CB or CR. Abbreviations: Sp, spine; Den, dendritic shaft. Scale bar, 0.5 μm.