. 2021 Jan 22;12(4):1175–1181. doi: 10.1021/acs.jpclett.0c03439

Table 1. Diffusion Coefficients D (mean ± standard deviation) for the Different Bilayers before and after HA Addition (see the Supporting Information) Measured on Glass Coverslips via Confocal FCS at Room Temperature^a.

lipid composition	D (μm²/s)	D (μm²/s) from ref (22)	D (μm²/s) in the presence of HA
DOPC	6.0 ± 0.3	6.3 ± 0.2	5.6 ± 0.4
DOPC/SM (1:1)	1.8 ± 0.4	2.6 ± 0.2	1.1 ± 0.6
DOPC/SM/Chol (2:2:1)	4.6 ± 0.9 (Ld); 0.4 ± 0.1 (Lo)^b	5.15 ± 0.15 (Ld); 0.255 ± 0.058 (Lo)	4 ± 2 (Ld); 0.3 ± 0.2 (Lo)^b

Values are compared to those reported in ref (22) in the absence of HA. For each bilayer composition before and after HA addition, 12–25 measurements were performed in three independent experiments.

The distinction between the macroscopic Lo and Ld domains is based on the fluorescence intensity differences of DiD, because this dye preferentially partitions into Ld regions.²⁵

Table 1. Diffusion Coefficients D (mean ± standard deviation) for the Different Bilayers before and after HA Addition (see the Supporting Information) Measured on Glass Coverslips via Confocal FCS at Room Temperaturea.

Table 1. Diffusion Coefficients D (mean ± standard deviation) for the Different Bilayers before and after HA Addition (see the Supporting Information) Measured on Glass Coverslips via Confocal FCS at Room Temperature^a.