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. 2021 Feb 4;49(2):0300060520986049. doi: 10.1177/0300060520986049

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Overexpression of senescence-associated lncRNA 1 (SAL-RNA1) upregulates sirtuin-1 (SIRT1) expression and suppresses p53 expression. AECIIs were transduced with the lentiviral vector LV-SAL-RNA1 and then treated with 10% cigarette smoke-media suspension (CSM) for 24 hours. Protein expression was assessed by western blotting. Bar graphs show densitometric analysis of blotting for SIRT1, forkhead box O3a (FoxO3a), p53, p21, matrix metalloprotease (MMP)-9, and tissue inhibitor of metalloprotease (TIMP)-1. Relative protein abundance was calculated using glyceraldehyde 3-phosphate (GAPDH) as the reference. Data represent the means ± standard deviations of three samples from a single experiment representative of three independent experiments. *p < 0.05 vs. senescent AECIIs. Groups: A (AECII control group), B (senescent AECII group), C (SIRT1 selective agonist SRT2104 group) and D (LV-SAL-RNA1 transduction group).