. 2021 Feb 8;22:54. doi: 10.1186/s12859-020-03901-y

Table 1.

Known HXB2 N-linked glycosylation site conservation by different alignment methods in HIV-1 protein B sequences (Bold value denotes equally maximum score and ^a when unique for our method)

HXB2 position	Location	NGlyAlign + Dialign	HIVAlign	ClustalW	Muscle	Clustal Omega	T-coffee
88 NVT	C1	100	100	98.72	100	100	100
136 NDT	V1	44.23	23.08	15.38	52.56	23.08	23.08
141 NSS	V1	55.13^a	17.95	26.28	7.05	6.41	20.51
156 NCS	V1	97.44	97.44	97.44	97.44	97.44	97.44
160 NIS	V2	89.1	89.1	89.1	89.1	89.1	89.1
186 NDT	V2	28.85^a	15.38	12.82	15.38	13.46	14.74
197 NTS	V2	98.08	98.08	98.08	98.08	98.08	98.08
230 NKT	C2	26.28	26.28	26.28	26.28	26.28	26.28
234 NGT	C2	74.36	74.36	74.36	74.36	74.36	74.36
241 NVS	C2	97.44	97.44	97.44	97.44	97.44	97.44
262 NGS	C2	100	100	100	100	100	100
276 NFT	C2	98.08	98.08	98.08	98.08	98.08	98.08
289 NTS	C2	69.23	69.23	69.23	69.23	69.23	69.23
295 NCT	C2	78.85	78.85	78.85	78.85	78.85	78.85
301 NNT	V3	92.95	92.95	92.95	92.95	92.95	92.95
332 NIS	C3	87.18	87.18	87.18	87.18	87.18	87.18
339 NNT	C3	77.56	77.56	77.56	77.56	77.56	78.21
356 NKT	C3	83.33	83.33	12.82	10.9	45.51	87.18
386 NST	V4	82.05	82.05	82.05	82.05	82.05	82.05
392 NST	V4	87.82	87.82	87.82	87.82	87.82	87.82
397 NST	V4	89.10^a	8.97	39.1	37.18	8.97	8.97
406 NNT	V4	32.05	20.51	17.31	50	8.97	16.03
448 NIT	C4	92.31	92.31	92.31	92.31	92.31	92.31
463 NES	V5	69.87^a	46.79	32.05	26.28	39.74	42.95
611 NAS	gp41	98.08	98.08	98.08	98.08	98.08	98.08
616 NKS	gp41	89.74	89.74	89.74	89.74	86.54	89.74
624 NHT	gp41	95.51	95.51	95.51	94.87	0.64	0.64
637 NYT	gp41	98.72	98.72	98.72	98.72	98.08	98.72
674 NIT	gp41	13.46	13.46	13.46	13.46	13.46	13.46
750 NGT	gp41	1.28	1.28	1.28	1.28	1.28	1.28
816 NAT	gp41	69.87	69.87	69.87	69.87	69.87	69.87
Combined score over N Sites		2317.95^a	2131.4	2069.87	2114.1	1962.82	2034.62