Fig. 1.

LiCl inhibits the survival and clonogenic potential of choroidal melanoma cells. a OCM1 and b M619 cells were seeded in 96-well plates, treated with 0, 2.5, 5, 10, 20 or 40 mM LiCl and incubated for 24 h, 36 h, 48 h. Cell survival was examined using the MTT assay. The survival rate at each drug concentration was compared with that of the normal saline group and analysed using SPSS software. All data are presented as the mean ± S.D. *P < 0.05,**P < 0.01,***P < 0.001. ns: not significant. c OCM1 and M619 cells were seeded in 6-well plates at a concentration of 1 × 10⁴ cells/well and cultured for approximately 2 weeks. Cells were treated with 0, 2.5, 5, or 10 mM LiCl, and various concentrations of LiCl were added to the wells every 72 h. When the cell colonies were visible to the naked eye, the culture was terminated, and the cells were stained with 1% crystal violet. d The size of the colonies. Magnification: × 100. Corresponding scale bars are depicted in the lower right corner of each image. Scale bars = 100 μm. e The number of clonies (> 50 cells) was counted under a microscope. f The diameter of a single colony was normalized to that in the vehicle-treated group. The experiments were repeated three times independently. All data are presented as the mean ± S.D. **P < 0.01, ***P < 0.001, ****P < 0.0001. NS not significant