Fig. 8.

Effects of the c.695_699del recessive mutation in DNAJB6 on its mRNA and protein stabilities in cells. a Lysates from HEK293 cell extracts transfected with 500 ng wild type M2-fused DNAJB6a (WT), 500 ng or 1500 ng M2-fused mutant DNAJB6a (500 ng mut, 1500 ng mut) plasmids, followed by immunoblot analysis with antibodies against M2 and β-actin. b Quantification of data in a. Data from three experiments (mean ± SEM). c RT-PCR analysis of WT or mutant DNAJB6a constructs transferred in HEK293 cells after actinomycin D (5 ug/ml) treatment for the indicated times points. d Western blot analysis of M2-fused WT or mutant DNAJB6a protein in HEK293 cells followed the treatment of cycloheximide (10 ug/ml) for the indicated time points. e Quantification of data in d. Data from three experiments (mean ± SEM). P value = * < 0.05, ** < 0.01. N.S. = not significant