. 2021 Feb 8;197:113956. doi: 10.1016/j.jpba.2021.113956

Table 2.

The effect of the PL removal step. Calibrants were prepared in human plasma, porcine plasma and water at the concentrations of 0.1, 2, 50, 100, 150, 200, 250 and 300 ng/mL. Linear calibration curves with concentration as an independent variable (y = ax + b) were created by weighted (1/x) regression analysis of the IS normalised peak areas. The average of two experiments is presented.

Calibrant matrix type	PL removal included	IS	a (± SD)	b (± SD)	R²
Human plasma	Yes	THC-OH D₃	0.0321 ± 0.0006	0.070 ± 0.096	0.998
Porcine plasma	Yes	THC-OH D₃	0.0321 ± 0.0012	0.152 ± 0.172	0.998
Water	Yes	THC-OH D₃	0.0324 ± 0.0005	0.111 ± 0.086	0.999
Human plasma	No	THC-OH D₃	0.0251 ± 0.0003	0.040 ± 0.048	0.999
Porcine plasma	No	THC-OH D₃	0.0321 ± 0.0006	0.132 ± 0.103	0.998
Water	No	THC-OH D₃	0.0364 ± 0.0005	0.073 ± 0.075	0.999
Human plasma	Yes	Diazepam D₅	0.0583 ± 0.0006	0.131 ± 0.099	0.999
Porcine plasma	Yes	Diazepam D₅	0.0584 ± 0.0015	0.153 ± 0.254	0.996
Water	Yes	Diazepam D₅	0.0588 ± 0.0008	0.091 ± 0.088	0.999
Human plasma	No	Diazepam D₅	0.0355 ± 0.0005	0.063 ± 0.078	0.999
Porcine plasma	No	Diazepam D₅	0.0464 ± 0.0005	0.097 ± 0.084	0.999
Water	No	Diazepam D₅	0.0538 ± 0.0007	0.061 ± 0.121	0.999