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. Author manuscript; available in PMC: 2021 Feb 8.
Published in final edited form as: Nat Genet. 2020 Feb 24;52(3):264–272. doi: 10.1038/s41588-020-0581-x

Extended Data Fig. 5: Validation of KO.

Extended Data Fig. 5:

a. Genotype confirmation by Sanger sequencing of the PCR products for all six successfully generated KO clones. b. PCR genotyping of KO derived mES clones to confirm deletion (deleted region on chromosome 9) in si-Δchr9 derived F1 and G9 clones, in triplicate (only representative results are shown here) in two independent experiments.. Additional primer R26 was designed to confirm heteroallelic deletion. Panel on the right determination of the gender of the KO clones are XY while wild type ES line is XX (refers to Methods). c. Genotyping by PCR to confirm deletion (deleted region on chromosome 7) in si-Δchr7 derived mES D4 and F4 clones.