KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-PAC	This paper	N/A
Mouse anti-FLAG	Sigma-Aldrich	Cat#F1804; RRID: AB_262044
Rabbit anti-EEA1	Cell Signaling Technology	Cat#3288; RRID: AB_2096811
Rabbit anti-LAMP1	Cell Signaling Technology	Cat#9091: RRID: AB_2687579
Rabbit anti-GAPDH	Cell Signaling Technology	Cat#2118; RRID: AB_561053
Alexa Fluor 546 Goat anti-mouse	Thermo Fisher Scientific	Cat# A11018; RRID: AB_1944229
Alexa Fluor 488 Goat anti-mouse	Thermo Fisher Scientific	Cat# A11017; RRID: AB_2107298
Chemicals, peptides, and recombinant proteins
Transferrin from human serum, biotin-xx	Thermo Fisher Scientific	Cat#T23363
Transferrin from human serum, Alexa Fluor 633 conjugate	Thermo Fisher Scientific	Cat#T23362
Transferrin from human serum, Alexa Fluor 488 conjugate	Thermo Fisher Scientific	Cat#T13342
Transferrin from human serum, fluorescein conjugate	Thermo Fisher Scientific	Cat#T2871
RIPA buffer	Sigma	Cat#R0278
Clensor	Saha et al., 2015	N/A
Intracellular pH Calibration Buffer Kit	Life Technologies	Cat#1787810
Experimental models: cell lines
Human: Human embryonic kidney (HEK293T) cells	ATCC	Cat#CRL-3216
Human: Flip-In T-REx 293 cells	Thermo Fisher Scientific	Cat#R78007
Human: HeLa cells	ATCC	Cat#CCL-2
Oligonucleotides
See Table S1 for list of oligonucleotides	This paper	N/A
Recombinant DNA
pcDNA5/FRT/TO PAC-FLAGC	This paper	N/A
pcDNA5/FRT/TO PAC-FLAG277	This paper	N/A
pmCherry-Cl Rab5-Q79L	Dr. Dejian Ren	N/A
Software and algorithms
GraphPad Prism	GraphPad Software	https://www.graphpad.com/; RRID:SCR_002798
pCLAMP 10.7	Molecular Devices	http://www.moleculardevices.com/products/software/pclamp.html; RRID:SCR_011323
Clampfit 10.7	Molecular Devices	N/A
Zen 2.3	ZEISS	http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen.html#introduction; RRID:SCR_013672
CorelDraw11	Corel Corporation	https://www.coreldraw.com/en/; RRID: SCR_014235
ImageJ	NIH	https://imagej.nih.gov/ij/