REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
COV2-2832	Zost et al., 2020a	N/A
COV2-2165	Zost et al., 2020a	N/A
COV2-2082	Zost et al., 2020a	N/A
rREGN10987	Starr et al., 2021	NA
r4A8	This paper	NA
FITC-conjugated chicken anti-cMyc antibody	Immunology Consultants Laboratory, Inc.	Cat# CMYC-45F
Alexa-647-conjugated Goat Anti-Human IgA+IgG+IgM	Jackson Immuno Research Laboratories	Cat# 109-605-064; RRID: AB_2337886
DyLight-405-conjugated Goat Anti-Human IgA+IgG+IgM	Jackson Immuno Research Laboratories	Cat# 109-475-064; RRID: AB_2337792
Goat anti-human IgG-Fc horseradish peroxidase (HRP)	Bethyl Labs	Cat# A80-104P; RRID: AB_67064
Biological samples
Normal Human Serum, collected 2017-2018 and pooled from 75 donors	Gemini Biosciences	Cat# 100–110, lot H86W03J
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 S2Pecto	Dingens et al., 2020	N/A
SARS-CoV-2 RBD	Dingens et al., 2020	NA
SARS-CoV-2 (COVID-19) Spike protein RBD-coupled magnetic beads	AcroBiosystems	Cat# MBS-K002
Critical commercial assays
Zymoprep Yeast Plasmid Miniprep II	Zymo Research	Cat# D2004
TMB/E HRP substrate	Millipore Sigma	Cat# ES001
Bright-Glo Luciferase Assay System	Promega	Cat# E2620
HIV-1 p24 Antigen Capture Assay	Advanced Bioscience Laboratories	Cat# 5421
Deposited data
PacBio CCSs linking variants to barcodes	Starr et al., 2020	NCBI SRA: BioProject PRJNA639956, BioSample: SAMN15295683
Illumina barcode sequencing	This paper	NCBI SRA: BioProject: PRJNA639956, BioSample: SAMN17185313
ACE2-bound RBD crystal structure	Lan et al., 2020	PDB: 6M0J
GISAID EpiCoV SARS-CoV-2 sequence isolates	GISAID	Full list of contributing labs and accessions: https://github.com/jbloomlab/SARS-CoV-2-RBD_MAP_HAARVI_sera/blob/main/data/gisaid_hcov-19_acknowledgement_table_2020_12_30.pdf
Experimental models: cell lines
Human: Embryonic Kidney (HEK293T)	ATCC	ATCC CRL-3216
Human: Embryonic Kidney cells expressing human ACE2 (HEK293T-hACE2)	BEI Resources	BEI NR-52511
Saccharomyces cerevisiae strain AWY101	Wentz and Shusta, 2007	AWY101
Recombinant DNA
Plasmid: pETcon_SARS-CoV-2_RBD	Starr et al., 2020	sequence at https://github.com/jbloomlab/SARS-CoV-2-RBD_DMS/tree/master/data/plasmid_maps/2649_pETcon-SARS-CoV-2-RBD-201aa.gb
Plasmid: HDM_Spikedelta21_D614G	Addgene	Addgene #158762
Plasmid: HDM-Hgpm2 gag/pol lentiviral helper plasmid	BEI Resources	BEI NR-52517
Software and algorithms
dms_variants, version 0.8.5	GitHub	https://jbloomlab.github.io/dms_variants/
dmslogo, version 0.6.2	GitHub	https://jbloomlab.github.io/dmslogo/
dms-view	Hilton et al., 2020	https://dms-view.github.io/docs/
neutcurve, version 0.5.2	GitHub	https://jbloomlab.github.io/neutcurve/
custom code	This paper	all analyses provided on github: https://github.com/jbloomlab/SARS-CoV-2-RBD_MAP_HAARVI_sera
Other
SARS-CoV-2 RBD mutant libraries	Starr et al., 2020	N/A
Infinite M1000Pro plate reader	Tecan	N/A
BD FACSAria II	BD Biosciences	N/A