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. 2020 Nov 5;30(11):1625–1638. doi: 10.1089/thy.2020.0105

FIG. 2.

FIG. 2.

PHGDH is essential for cell proliferation and tumorigenesis. (A, B) Cell proliferation and colony formation were assessed in PHGDH knockdown thyroid cancer cells. (A) The relative number of viable cells was significantly decreased with PHGDH knockdown in BCPAP and 8505C cells at day 6 (p < 0.05). (B) PHGDH knockdown also significantly inhibited the colony formation of 8505C cells (p < 0.01). (C, D) Cell proliferation and colony formation were assessed after PHGDH inhibitor (NCT503) treatment for thyroid cancer cells. (C) Treatment with NCT503 (15 μM) significantly inhibited cell growth at days 3 to 5 in BCPAP and 8505c cells. (D) NCT503 (15 μM) treatment significantly inhibited colony formation of BCPAP and 8505c cells at day 7. (E) Tumorsphere formation was assessed after PHGDH knockdown. shPHGDH #2-transfected BCPAP cells showed a significant decrease in the number of tumorspheres larger than 50 μm measured at day 10 (p < 0.05). (F) NCT503 (15 μM) treatment also significantly inhibited tumorsphere formation with a decreased number of tumorspheres larger than 50 μm, measured at day 10 in both BCPAP and 8505c cells (p < 0.01). Data are expressed as mean ± SD of 3 independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001. shRNA, small hairpin RNA. Color images are available online.