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. 2021 Jan 14;8:620629. doi: 10.3389/fbioe.2020.620629

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Copyright © 2021 Xu, Qian, Guan, Ren, Yang, Huang, Zhang, Chai, Wu, Wu, Zhang, Yang and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Copper-containing metal (CCM) accelerated callus formation in bone repair. (A) HandE-stained histological images of the tibia of 316L/316L−5Cu-inserted mice 7, 14, and 21 days after injury. Yellow dotted lines indicate the edge of the drill hole. Scale bar represents 200 μm. (B) Three-dimensional microcomputed tomography (μCT) images of the drill holes in 316L/316L−5Cu-inserted mice 7, 14, and 21 days after injury. The ratio of bone volume to tissue volume (BV/TV), representing bone formation in the drill holes, was calculated. Data were statistically analyzed by Student's t-test. Results are presented as mean ± SEM. n = 6 per time point per group.