Table 2. Observations made for the batch micro-crystallization of AcNiR using different protein:precipitant ratios and thus different ammonium sulfate concentrations.

Protein:precipitant ratio	1:1	1:2	1:3
Crystals	No	15–20 µm	15–20 µm
Precipitate	Light	Moderate	Heavy
Crystallization time	N/A	4–5 days (no seeds)	3–4 days (no seeds)
		1–2 days (seeds)	Overnight (seeds)