Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 26;77(Pt 2):237–248. doi: 10.1107/S2059798320016125

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Moosmayer et al. 2021

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

PMC Copyright notice

(a) Comparison of the effect of different SECIS elements on GPX4 expression when co-expressed with rat SBP2 using transient transfection in HEK293-6E cells. Transfected cells were lysed and GPX4 was partially purified by IMAC, and the eluates were analyzed by SDS–PAGE. Lanes 1–4 show expression of GPX4 with the chimeric SECIS (lane 1), with SelN SECIS (lane 2), with GPX4 SECIS (lane 3) and with T. gondii SECIS (lane 4). Lane 5 shows Mock-transfected HEK293-6E cells. The arrow indicates the band representing GPX4^WT (molecular weight 21 155 Da). (b) Purification steps for GPX4^WT analyzed by SDS–PAGE. Lane 1 shows the pooled fractions after IMAC as subjected to gel filtration; lane 2 shows the pooled fractions of the final gel-filtration step. (c) Mass-spectrometric analysis of purified GPX4^WT is consistent with 100% incorporation of one Se atom. The deconvoluted spectrum is shown.