Characterization of single-cell T cells from mass cytometry data. A: t-distributed stochastic neighbor embedding (t-SNE) plot of major T-cell subsets. Cells are colored on the basis of cell types. B: violin plot showing the expression of CD4, CD28, CD27, and CD161 in the T-cells cluster between the healthy control (HC), acute phase (AP), and convalescent phase (CP) group. C: t-SNE projections of major T-cell subsets from the HC, AP, and CP group, respectively. D: percentage of major T-cell subsets in all blood CD45+ immune cells from HC (n = 5), AP (n = 5), and CP group (n = 4). E: relative fractions of major T-cell subsets in T cells from the HC, AP, and CP group. Adjusted P values are based on Wilcoxon rank-sum test (marker level) or two-way ANOVA tests with Bonferroni’s post hoc correction (parametric) between groups. ****Significant with adjusted P value < 0.0001. **Significant with adjusted P value < 0.01. *Significant with adjusted P value < 0.05.