Fig 4. Immunostaining, food intake, body weight, and circadian behavior of mice with constitutive deletion of D1R (D1R KO^KOMP).

Forebrain staining of (A) control TH (pink) and DAPI (blue), (B) D1R KO^KOMP TH and DAPI, (C) control D1R (green) and DAPI, (D) D1R KO^KOMP and DAPI (E) control merge and (F) D1R KO^KOMP merge. (G) Average daily food intake for control (n = 21) and D1R KO^KOMP (n = 18) mice in grams and (H) normalized to body weight. (I) Body weight in grams prior to CR and during 4 weeks of CR and (J) expressed as a fraction of Day 0 weight. (K) Mean ± SEM fraction of high activity behaviors on day 0 of CR. n = 18–22 for control and 16–19 for KO for all behavioral measurements. (L) Mean ± SEM fraction of high activity behaviors after 14 days of timed CR feeding. (M) Mean ± SEM fraction of high activity behaviors after 28 days of timed CR feeding. (N) Mean ± SEM total 24 hour high activity data in seconds. (O) Mean ± SEM high activity data in seconds for the 3 h preceding scheduled feeding across the experiment (sum of ZT 5, 6, and 7). (P) Normalized high activity data shown in (O) in the 3 h preceding scheduled feeding across the experiment.