Fig 2. Generation of chikungunya DVGs in mosquitoes in vivo.

(A) Aedes aegypti mosquitoes were fed a blood meal infected with 10⁶ PFU/ml of CHIKV Carib. After 7 days, ten mosquitoes were sacrificed and midgut, abdominal wall (body), thorax, head and legs/wings were dissected. After verification of infection by titration, viral RNA was extracted and deep-sequenced. (B) Analysis of the deletions generated by CHIKV Carib in nine infected mosquitoes pooled together. Start (x axis) and stop (y axis) positions of the breakpoint of DVGs generated are plotted for each organs. (C) Start (x axis) and stop (y axis) positions of the breakpoint of DVGs generated in the different organs of 2 individual mosquitoes. The red dots represent the deletion starting at 2695 and ending at 5358, this DVG is called CM1. The number next to it is the number of read per million reads (RPM).