Fig 5. Defective viral genomes prevent viral dissemination of chikungunya virus in Aedes aegypti mosquitoes.

(A) 150 ng of DVG candidate RNA (IH1, CV4 or CM1), a control RNA or PBS were injected into Aedes aegypti mosquitoes 2 days prior to being fed an infected blood meal containing 10⁶ PFU/ml. After 5 days, mosquitoes were sacrificed, and midguts were separated from carcasses. (B) Luciferase activity of midgut or carcass of each mosquito was measured. (C) After virus titration, the proportions of positive midguts or carcasses (PFU≥100 PFU/ml) were determined. (Representative result of one experiment out of two independent experiments is shown, n = 24, *p<0,05, **p<0,01, ***p<0,001, ns non significant compared to PBS after one-way ANOVA with multiple comparison (B) or Chi square test, multiple comparison with Holm correction (C)).