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. 2021 Feb 8;16(2):e0246422. doi: 10.1371/journal.pone.0246422

Fig 4. Comparison of the mucosal adjuvant effects of the enzymatically polymerised polyphenols with CT.

Fig 4

Female BALB/cCrSlc mice were immunised three times intranasally (days 0, 7, and 14) with OVA (2.5 μg/mouse) plus either pCA (100 μg/mouse), pFA (100 μg/mouse), or pCoA (100 μg/mouse), or OVA (2.5 μg/mouse) plus CT (1 μg/mouse). ELISA detected OVA-specific IgA endpoint titres in nasal wash and BALF; IgG endpoint titres in BALF and vaginal wash; and total IgG, IgG1, and IgG2a endpoint titres in sera on day 21. The data were obtained from three biologically independent experiments. OVA plus enzymatically polymerised polyphenols (EPPs); the median value of the sum of OVA plus pCA, OVA plus pFA, and OVA plus pCoA, n = 27; OVA plus CT, n = 9. The box-plot shows the median value with the 25th–75th percentiles and the error bars indicate the 5th–95th percentiles. Significance was calculated using the Mann-Whitney U test: *p < 0.05.