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. Author manuscript; available in PMC: 2022 Feb 5.
Published in final edited form as: Circ Res. 2020 Dec 2;128(3):335–357. doi: 10.1161/CIRCRESAHA.120.316738

Fig. 4.

Fig. 4.

A: Immunoblots of cardiac PPARα and β-ACTIN proteins in non-diabetic, diabetic KLF5fl/fl and diabetic αMHC-Klf5−/− mice 12 weeks post-STZ administration (analysis shown in Online Figure XVII). B: Immunoblots of PPARα and β-ACTIN proteins in AC16 cells infected with adenoviruses expressing GFP, wtFOXO1 or caFOXO1 (analysis shown in Online Figure XIX–B). C: FOXO1 enrichment in PPARα promoter sequence fragments precipitated with FOXO1 antibody from AC16 cells expressing GFP or wtFOXO1. D: Immunoblot of cardiac KLF5 and β-ACTIN proteins in C57BL/6 mice infected with AAV9-cTnT or AAV9-cTnT-hKlf5 (analysis shown in Online Figure XXIV–A), and immunoblot of cardiac KLF5 and total protein stain of diabetic FOXO1fl/fl mice and diabetic αMHC-FoxO1−/− mice infected with control AAV9-cTnT or AAV9-cTnT-hKlf5 (analysis shown in Online Figure XXIV–B). E: Left ventricular fractional shortening of C57BL/6 mice infected with AAV9-cTnT or AAV9-cTnT-hKlf5 2, 4, 6 and 8 weeks post-AAV9 administration. F: Left ventricular fractional shortening of diabetic FOXO1fl/fl mice and diabetic αMHC-FoxO1−/− mice infected with AAV9-cTnT or AAV9-cTnT-hKlf5. G: Immunoblot of cardiac KLF5 and PonceauS staining of lsl-rtTA-TRE-Klf5 and αMHC-rtTA-Klf5 mice fed on doxycycline enriched diet for 2 weeks (analysis shown in Online Figure XXV–D). H: Left ventricular fractional shortening of lsl-rtTA-TRE-Klf5 and αMHC-rtTA-Klf5 mice fed on doxycycline enriched diet for 2 and 4 weeks.