Table 1.

Timing information for post-PCR to sequencing. NA: Not applicable

	Step	MinION	Flongle
Library Preparation	Post-Amplification DNA prep	65 min	15 min
	DNA End prep	2.0 h	37 min
	Native barcode ligation	2.5 h	NA
	Adapter Ligation and Clean-up	1.0 h	52 min
	Total Library Prep Time	6.5 h	1.75 h
Sequencing & Analysis	Flow cell check	15 min	10 min
	Priming and loading flow cell	10 min	5 min
	Sequencing	4.6–18.4 h	1.0 h
	Base calling	2.5 h	4.5 min
	Demultiplexing	17 min	NA
	Consensus	5.4 h (13.5 min/sample)	11.5 min
	Genotyping	1.0 h (2.5 min/sample)	2.1 min
	Total Sequencing & Analysis Time	14.2–28.0 h	1.5 h
Total Time		20.6–34.4 h	3.3 h