Table 2.
MinION sequencing experiment metrics.
| Sequencing Run | Total run time (h) |
Number of active pores |
Total # of reads per run (millions) |
Number of reads per hour (thousands) |
Total reads analyzed |
Time to reach 800 k reads (h) |
Total # of reads assigned to barcodes |
Number of samples |
Average # of reads per sample (demultiplexed) |
Average # of usable reads per sample |
Average # of reads per locus |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 16.6 | 1,714 | 2.78 | 167.4 | 800,000 | 4.42 | 722,199 | 24 | 30,092 | 17,125 | 1,818 |
| 2 | 4.6 | 1,267 | 0.83 | 180.4 | 800,000 | 4.45 | 685,527 | 24 | 28,564 | 13,827 | 1,474 |
| 3* | 18.4 | 1,225 | 2.51 | 136.4 | 800,000 | 4.57 | 679,949 | 24 | 29,170 | 14,510 | 1,611 |
| 4 | 6.1 | 1,460 | 1.01 | 165.6 | 800,000 | 4.70 | 701,794 | 24 | 29,241 | 16,131 | 1,661 |
| 5 | 15.6 | 1,688 | 3.28 | 210.3 | 800,000 | 3.28 | 723,768 | 24 | 30,157 | 11,795 | 1,281 |
| Average | 12.3 | 1,471 | 2.08 | 169.8 | 800,000 | 4.28 | 702,647 | 24 | 29,445 | 14,678 | 1,569 |
*
Two samples failed to amplify on this run. Upon repeat the samples were successfully sequenced.