Table 4.
Multiplexed PCR Method | Sequencing Run | Total Run Time (min) | Number of Active Pores | Total Reads per run | Number of Usable reads | Average reads per locus |
---|---|---|---|---|---|---|
NGSgo®-MX6-1 | 1 | 60 | 76 | 25,000 | 10,289 | 1,715 |
2 | 60 | 85 | 28,000 | 13,473 | 2,246 | |
3 | 60 | 71 | 30,000 | 17,195 | 2,866 | |
4 | 60 | 74 | 24,000 | 13,504 | 2,251 | |
5 | 60 | 83 | 33,000 | 17,116 | 2,853 | |
6 | 60 | 90 | 36,000 | 18,597 | 3,100 | |
7 | 60 | 77 | 24,000 | 16,001 | 2,667 | |
8 | 60 | 58 | 19,000 | 13,745 | 2,291 | |
9 | 60 | 73 | 23,000 | 13,130 | 2,188 | |
Average | 76 | 26,889 | 14,783 | 2,464 | ||
OmniType | 1 | 60 | 63 | 15,000 | 6,418 | 642 |
2 | 60 | 84 | 21,000 | 13,035 | 1,448 | |
3 | 60 | 78 | 21,000 | 13,155 | 1,462 | |
4 | 60 | 68 | 17,000 | 9,623 | 1,069 | |
5 | 60 | 86 | 23,000 | 13,789 | 1,532 | |
6 | 60 | 92 | 29,000 | 15,605 | 1,734 | |
7 | 60 | 67 | 14,000 | 8,102 | 810 | |
8 | 60 | 66 | 16,000 | 9,480 | 948 | |
9 | 60 | 79 | 20,000 | 11,699 | 1,170 | |
Average | 76 | 19,556 | 11,212 | 1,202 |