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. Author manuscript; available in PMC: 2021 Feb 8.
Published in final edited form as: Hum Immunol. 2020 Jun 25;81(8):413–422. doi: 10.1016/j.humimm.2020.06.004

Table 4.

Flongle Sequencing Metrics.

Multiplexed PCR Method Sequencing Run Total Run Time (min) Number of Active Pores Total Reads per run Number of Usable reads Average reads per locus
NGSgo®-MX6-1 1 60 76 25,000 10,289 1,715
2 60 85 28,000 13,473 2,246
3 60 71 30,000 17,195 2,866
4 60 74 24,000 13,504 2,251
5 60 83 33,000 17,116 2,853
6 60 90 36,000 18,597 3,100
7 60 77 24,000 16,001 2,667
8 60 58 19,000 13,745 2,291
9 60 73 23,000 13,130 2,188
Average 76 26,889 14,783 2,464
OmniType 1 60 63 15,000 6,418 642
2 60 84 21,000 13,035 1,448
3 60 78 21,000 13,155 1,462
4 60 68 17,000 9,623 1,069
5 60 86 23,000 13,789 1,532
6 60 92 29,000 15,605 1,734
7 60 67 14,000 8,102 810
8 60 66 16,000 9,480 948
9 60 79 20,000 11,699 1,170
Average 76 19,556 11,212 1,202