Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (MbA_g2490) | g2490 | This paper | ||
| Strain, strain background (Escherichia coli) | DH5α | Other | Doehlemann lab | |
| Genetic reagent (Pichia pastoris) | KM71H-OCH | Other | Doehlemann lab | |
| Antibody | Monoclonal 6x-His tag antibody | Sigma (St. Louis; Mississippi; USA) | 1/10,000 | |
| Antibody | Mouse IgG (Monoclonal) |
Thermo Fischer Scientific (Waltham; Massachusetts; USA) | 1/3000 | |
| Recombinant DNA reagent | pGAPzα (plasmid) | Invitrogen, Carlsbad, CA, USA | ||
| Sequence-based reagent | A. thaliana EF1-α:forward | Ruhe et al., 2016 | PCR primers | AAGGAGGCTGCTGAGATGAA |
| Sequence-based reagent | A. thaliana EF1-α:reverse | Ruhe et al., 2016 | PCR primers | TGGTGGTCTCGAACTTCCAG |
| Sequence-based reagent | Oomycete internal transcribed spacer (ITS) 5.8 s: forward | Ruhe et al., 2016 | PCR primers | ACTTTCAGCAGTGGATGTCTA |
| Sequence-based reagent | Oomycete internal transcribed spacer (ITS) 5.8 s: reverse | Ruhe et al., 2016 | PCR primers | GATGACTCACTGAATTCTGCA |
| Commercial assay or kit | EnzChek Lysozyme Assay Kit | Invitrogen | E22013 |
Lysozyme activity assay |
| Chemical compound, drug | Trypan blue stain | Sigma Aldrich (No. 302643) |
CAS-Number: 72-57-1 |