Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | Human iPS cell line | Riken BRC Cellbank | hiPS-409-B2 | Female |
| Cell line (Homo sapiens) | Human iPS cell line stable transfected with Ngn2 and rttA | This paper | hiPS-409-B2_Ngn2 | Generated for this study. See Materials and methods. |
| Cell line (Homo sapiens) | Human iPS cell line | Systems Biosciences | SC102A-1 | Male |
| Cell line (Homo sapiens) | Human iPS cell line stable transfected with Ngn2 and rttA | This paper | SC102A-1_Ngn2 | Generated for this study. See Materials and methods. |
| Cell line (Homo sapiens) | Human iPS cell line | This paper | HmRNA | Generated for this study. See Materials and methods. Female Reprogramming by mRNA |
| Cell line (Homo sapiens) | Human iPS cell line stable transfected with Ngn2 and rttA | This paper | HmRNA_Ngn2 | Generated for this study. See Materials and methods. |
| Cell line (Pan troglodytes) | Chimpanzee iPS cell line | Generated in a previous study (Mora-Bermúdez et al., 2016) | Sandra_A | Female |
| Cell line (Pan troglodytes) | Chimpanzee iPS cell line stable transfected with Ngn2 and rttA | This paper | Sandra_A_Ngn2 | Generated for this study. See Materials and methods. |
| Cell line (Pan troglodytes) | Chimpanzee iPS cell line | Generated in a previous study (Mora-Bermúdez et al., 2016) | Jo_C | Male |
| Cell line (Pan troglodytes) | Chimpanzee iPS cell line stable transfected with Ngn2 and rttA | This paper | Jo_C_Ngn2 | Generated for this study. See Materials and methods. |
| Cell line (Pan troglodytes) | Chimpanzee iPS cell line | Provided by the Max-Delbrück-Centrum für Molekulare Medizin | ciPS01 Chimp male iPSC Sendai CL5 |
Male |
| Cell line (Pan troglodytes) | Chimpanzee iPS cell line stable transfected with Ngn2 and rttA | This paper | ciPS01_Ngn2 | Generated for this study. See Materials and methods. |
| Cell line (Pan paniscus) | Bonobo iPS cell line | Generated for a previous study (Kanton et al., 2019) | BmRNA | Female Reprogramming by mRNA |
| Cell line (Pan paniscus) | Bonobo iPS cell line stable transfected with Ngn2 and rttA | This paper | BmRNA_Ngn2 | Generated for this study. See Materials and methods. |
| Antibody | MAP2 chicken polyclonal antibody | Invitrogen | PA1-16751 | Dilution (1:1000) |
| Antibody | SYN1/2 guinea pig polyclonal antibody | Synaptic Systems | 106004 | Dilution (1:1000) |
| Antibody | TUBB3 mouse monoclonal antibody | BioLegend | 801202 | Dilution (1:1000) Coupled to AlexaFlour 488 or Alexa Flour 555 |
| Antibody | Purified anit-Neurofilament Marker (pan-axonal, cocktail) mouse monoclonal antibody | BioLegend | 8379074 | Dilution (1:400) Clone: SMI312 |
| Antibody | ISL1 mouse monoclonal antibody | Invitrogen | MA5-15515 | Dilution (1:1000) |
| Antibody | PRPH chicken polyclonal antibody | Abcam | ab39374 | Dilution (1:1000) |
| Antibody | BRN2 goat polyclonal antibody | Santa Cruz | sc-6029 | Dilution (1:100) |
| Antibody | TBR1 rabbit polyclonal antibody | Abcam | Ab31940 | Dilution (1:200) |
| Antibody | CUX1 mouse monoclonal antibody | Santa Cruz | sc-514008 | Dilution (1:500) |
| Recombinant DNA reagent | pmax GFP (plasmid) | Lonza | D-00072 | |
| Recombinant DNA reagent | pLVX-EF1α-(Tet-On-Advanced)-IRES-G418(R) (plasmid) |
Provided by Nael Nadif Kasri (Frega et al., 2017) | This vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418. | |
| Recombinant DNA reagent | pLVX-(TRE-thight)-(MOUSE)Ngn2-PGK-Puromycin(R) (plasmid) |
Provided by Nael Nadif Kasri (Frega et al., 2017) | This vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter. | |
| Commercial assay or kit | Stem MACS mRNA transfection Kit | Miltenyi Biotec | 130-104-463 | |
| Commercial assay or kit | Human Pluripotent Stem Cell 3 Colour Immunohistochemistry Kit | R and D Systems1 | SC021 | |
| Commercial assay or kit | Human Pluripotent Stem Cell Functional Identification Kit | R and D Systems | SC027B | |
| Commercial assay or kit | StemMACS Trilineage Differentiation Kit | Miltenyi Biotec | 130-115-660 | |
| Software, algorithm | Imaris 9.5 | Bitplane | ||
| Software, algorithm | Origin | OriginLab version 2018-2019b | ||
| Software, algorithm | Patch and Fitmaster software | HEKA version 2.9x | ||
| Software, algorithm | Custom script to analyse neuron morphology based on Imaris raw files (.hoc) | This paper (Peter and Schörnig, 2020) | For quantification, we exported Sholl intersections and .hoc files, as they contain information about both the neurite length and the branching pattern. HOC files were analyzed using a custom script. See Materials and methods. (Peter and Schörnig, 2020) | |
| Software, algorithm | R 3.5.1 | R |