Figure 2.

Rapamycin decreases PTSC senescence. (a) Treatment of PTSCs with rapamycin reduces cell proliferation. Data are presented as the mean ± SD, triplicates. ^∗p < 0.01 compared to the control group without rapamycin treatment. (b) Treatment of PTSCs with 50 μg/ml bleomycin induces β-gal, but it is abolished by combined treatment with 25 nM rapamycin for 5 days. Scale bar = 100 μm. (c) Semiquantification confirms that rapamycin blocks β-gal induced by bleomycin. Results are expressed as mean ± SD, n = 3. ^∗p < 0.01, compared to the bleomycin treatment group. Cont: control; Bleo: bleomycin; Rapa: rapamycin.