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. 2021 Feb 1;2021:6658944. doi: 10.1155/2021/6658944

Figure 2.

Figure 2

Fenofibrate reduced the expression of inflammatory cytokines. (a) Serum IL-1β and TNF-α were detected by ELISA. (b) Relative IL-1β and TNF-α mRNA levels were determined by qRT-PCR. (c) Western blot analysis of TNF-α, IL-6, and IL-1β protein levels. The western blot results were quantified with ImageJ 8.0 software. (d) Immunohistochemical staining (200x) showed expression of IL-1β and TNF-α protein in liver tissues at 8 hours after reperfusion. Final evaluations were made using Image-Pro Plus 6.0 software to calculate the IOD of the positive staining area. Data are presented as mean ± SD (n = 6; ∗P < 0.05 for IR vs. sham; #P < 0.05 for IR+FF (50) vs. IR; +P < 0.05 for IR+FF (100) vs. IR; ^P < 0.05 for IR+FF (50) vs. IR+FF (100)).