Table 1.
Oligonucleotide sequences of primers used for qRT-PCR.
| Gene | DNA strand | Primer sequence (5′-3′) |
|---|---|---|
| β-Actin | Forward | GGCTGTATTCCCCTCCATCG |
| Reverse | CCAGTTGGTAACAATGCCATGT | |
| TNF-α | Forward | CAGGCGGTGCCTATGTCTC |
| Reverse | CGATCACCCCGAAGTTCAGTAG | |
| IL-1β | Forward | GAAATGCCACCTTTTGACAGTG |
| Reverse | TGGATGCTCTCATCAGGACAG | |
| Bax | Forward | AGACAGGGGCCTTTTTGCTAC |
| Reverse | AATTCGCCGGAGACACTCG | |
| Bcl-2 | Forward | GCTACCGTCGTCGTGACTTCGC |
| Reverse | CCCCACCGAACTCAAAGAAGG | |
| Casepase-9 | Forward | GGCTGTTAAACCCCTAGACCA |
| Reverse | TGACGGGTCCAGCTTCACTA | |
| Casepase-3 | Forward | CTCGCTCTGGTACGGATGTG |
| Reverse | TCCCATAAATGACCCCTTCATCA | |
| Beclin-1 | Forward | ATGGAGGGGTCTAAGGCGTC |
| Reverse | TGGGCTGTGGTAAGTAATGGA | |
| LC3 | Forward | GACCGCTGTAAGGAGGTGC |
| Reverse | AGAAGCCGAAGGTTTCTTGGG | |
| P62 | Forward | GAGGCACCCCGAAACATGG |
| Reverse | ACTTATAGCGAGTTCCCACCA | |
| PPAR-α | Forward | AACATCGAGTGTCGAATATGTGG |
| Reverse | CCGAATAGTTCGCCGAAAGAA | |
| p-AMPK | Forward | ATTGGATTTCCGAAGTATTGATG |
| Reverse | CCTGGTCTTGGAGCTACGTCA |