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. 2020 Nov 24;83(1):108–111. doi: 10.1292/jvms.20-0403

Table 1. Primary antibodies used in the present case.

Antibody Clone Dilution Source Antigen retrieval Positive control tissue (Asian palm civet)
Pan-Cytokeratin AE1/AE3 1:300 Abcam, Cambridge, MA, USA Microwave (100°C) for 15 min in citrate buffer, pH 6.0 Epidermis and adnexa of the skin

Vimentin V9 1:300 Abcam Microwave (100°C) for 15 min in citrate buffer, pH 6.0 Macrophages and smooth muscle in the lung

Iba-1 20A12.1 1:400 Millipore, Billerica, MA, USA Microwave (100°C) for 15 min in citrate buffer, pH 6.0 Alveolar macrophages in the lung

CD 204 Polyclonal antibody 1:300 Abcam Microwave (100°C) for 20 min in citrate buffer, pH 6.0 Alveolar macrophages in the lung

HLA-DR LN3 1:300 Novocastra, Chicago, IL, USA Microwave (100°C) for 30 min in citrate buffer, pH 6.0 Subcutaneous macrophages

Smooth muscle actin 1A4 1:300 Cell Marque, St. Louis, MO, USA Microwave (100°C) for 20 min in citrate buffer, pH 6.0 Smooth muscle of the bronchioles