| Pan-Cytokeratin |
AE1/AE3 |
1:300 |
Abcam, Cambridge, MA, USA |
Microwave (100°C) for 15 min in citrate buffer, pH 6.0 |
Epidermis and adnexa of the skin |
|
| Vimentin |
V9 |
1:300 |
Abcam |
Microwave (100°C) for 15 min in citrate buffer, pH 6.0 |
Macrophages and smooth muscle in the lung |
|
| Iba-1 |
20A12.1 |
1:400 |
Millipore, Billerica, MA, USA |
Microwave (100°C) for 15 min in citrate buffer, pH 6.0 |
Alveolar macrophages in the lung |
|
| CD 204 |
Polyclonal antibody |
1:300 |
Abcam |
Microwave (100°C) for 20 min in citrate buffer, pH 6.0 |
Alveolar macrophages in the lung |
|
| HLA-DR |
LN3 |
1:300 |
Novocastra, Chicago, IL, USA |
Microwave (100°C) for 30 min in citrate buffer, pH 6.0 |
Subcutaneous macrophages |
|
| Smooth muscle actin |
1A4 |
1:300 |
Cell Marque, St. Louis, MO, USA |
Microwave (100°C) for 20 min in citrate buffer, pH 6.0 |
Smooth muscle of the bronchioles |