Table 1.

Oligonucleotide sequences of primers and probes used in this study (Centers for Disease Control and Prevention, 2020).

Assay	Target gene	Primer/probe	Sequence (5′–3′)
CDC N1	Nucleocapsid (N)	2019-nCoV_N1-F	GACCCCAAAATCAGCGAAAT
		2019-nCoV_N1-R	TCTGGTTACTGCCAGTTGAATCTG
		2019-nCoV_N1-P	FAM-ACCCCGCATTACGTTTGGTGGACCa
CDC N2	Nucleocapsid (N)	2019-nCoV_N2-F	TTACAAACATTGGCCGCAAA
		2019-nCoV_N2-R	GCGCGACATTCCGAAGAA
		2019-nCoV_N2-P	FAM-ACAATTTGCCCCCAGCGCTTCAGa
CDC RP	Human RNase P	RNAse P-F	AGATTTGGACCTGCGAGCG
		RNAse P-R	GAGCGGCTGTCTCCACAAGT
		RNAse P-P	FAM–TTCTGACCTGAAGGCTCTGCGCGa

^aFAM, 6-carboxyfluorescein;