Table 1.
Assay | Target gene | Primer/probe | Sequence (5′–3′) |
---|---|---|---|
CDC N1 | Nucleocapsid (N) | 2019-nCoV_N1-F | GACCCCAAAATCAGCGAAAT |
2019-nCoV_N1-R | TCTGGTTACTGCCAGTTGAATCTG | ||
2019-nCoV_N1-P | FAM-ACCCCGCATTACGTTTGGTGGACCa | ||
CDC N2 | Nucleocapsid (N) | 2019-nCoV_N2-F | TTACAAACATTGGCCGCAAA |
2019-nCoV_N2-R | GCGCGACATTCCGAAGAA | ||
2019-nCoV_N2-P | FAM-ACAATTTGCCCCCAGCGCTTCAGa | ||
CDC RP | Human RNase P | RNAse P-F | AGATTTGGACCTGCGAGCG |
RNAse P-R | GAGCGGCTGTCTCCACAAGT | ||
RNAse P-P | FAM–TTCTGACCTGAAGGCTCTGCGCGa |
FAM, 6-carboxyfluorescein;