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. 2021 Jan 26;11:606386. doi: 10.3389/fpls.2020.606386

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Copyright © 2021 Besnard, Sonawala, Maharjan, Collakova, Finlayson, Pilot, McDowell and Okumoto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Constitutive expression of UMAMIT14 leads to a stunted phenotype. (A) Macroscopic phenotypes of 3-week-old Arabidopsis plants from T2 generation, expressing UMAMIT1-cMyc fusion under 35S promoter, compared to the wild type plant (right). Scale bar: 1 cm. (B) Top: Western blot targeting UMAMIT14-c-myc from corresponding lines shown in (A). Each lane contained 10 μg of proteins extracted from 4-week-old wild type and 35S:UMAMIT14 leaves. Arrowhead indicates 40 kDa. Bottom: RubisCO protein visualized with Ponseau S staining of the membrane used for the Western blot shown in the top panel.