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. 2021 Jan 26;8:630246. doi: 10.3389/fchem.2020.630246

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Copyright © 2021 Ding, Zhou, Guo and Su.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bright field (BF, A) and ECL (B–F) images of gold-coated UMEA in PB (0.1 M, pH 7.4) containing 50 μM Ru(bpy)₃ ²⁺ and 50 mM TPrA. The ECL imaging were triggered by a double-step potential (initial potential 0 V; respective pulse potentials 0.9, 1.0, 1.1, 1.2, 1.3 V; pulse period 2 s; pulse time 1 s). The exposure time of EMCCD was 4 s. (G) The grayscale variation of ECL along the radial direction of two adjacent microwells. (H) The variation of ECL intensity with the potential at the gold-coated UMEA at single microwell levels.