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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Bioorg Chem. 2020 Dec 30;107:104595. doi: 10.1016/j.bioorg.2020.104595

Figure 6: Potent flavonol analogs inhibit the protein expressions of activated c-Kit and downstream effectors levels, including phosphorylated mTOR/ ribosomal protein S6/ MAPK (ERK1/2) and p90RSK in A375 melanoma and A431 non-melanoma cells.

Figure 6:

(A) Western blots displays are representatives of c-Kit downstream effectors the protein expression levels of phosphorylated mTOR, ribosomal protein S6, MAPK p44/42(ERK1/2) and p90RSK, in 48h treated A375 (left panel) and A431 (right panel) cells. A375/A431 cells were incubated with/without test compounds (F20; 0, IC50, 2×IC50; μM, 48h), and western blotting was carried out as described in the Methods section. Equal protein loading was confirmed by reprobing for Rab11 as loading control, and protein levels were normalized to the loading control and expressed as percentage. The data shown are representative of immunoblot of three independent experiments with similar results. (B) The data expressed in the bar graphs represent mean ± SD of relative quantitative normalized density values in percentage with an internal loading control. The analogs F20 significantly suppressed the protein expression levels of the phorphorylated form of the targets in A375 and A431 as compared with untreated control cells. Statistical significance was determined using one-way ANOVA and Dunn’s multiple comparison test, and p < 0.05 (*) was considered significant.